Functional coupling between A1 adenosine receptors and G-proteins in rat hippocampal membranes assessed by high-affinity GTPase activity

British Journal of Pharmacology
Y Odagaki, K Fuxe

Abstract

1. In order to investigate the functional interaction between adenosine receptors and G-proteins in native brain membranes, stimulation of high-affinity GTPase activity by adenosine receptor agonists was characterized in rat hippocampal membranes. 2. Addition of 1 microM R-N6-phenylisopropyladenosine (R-PIA), a selective A1 adenosine receptor agonist, augmented the Vmax of the low-KM GTPase by 51%, with a slight increase in the KM value. 3. Several adenosine receptor agonists stimulated the high-affinity GTPase activity in a concentration-dependent manner, with a rank order of potency indicative of the involvement of A1 adenosine receptor subtype as follows: R-PIA > N6-cyclohexyladenosine > 5'-N-ethylcarboxamidoadenosine > or = 2-chloroadenosine > S-PIA > CGS 21680, 2-phenylaminoadenosine. 4. The selective A1 adenosine receptor antagonist, 8-cyclopentyl-1,3-dipropylxanthine, inhibited R-PIA-stimulated high-affinity GTPase activity in a competitive manner, with a KB value of 2.5 nM. 5. The activating effects on high-affinity GTPase of R-PIA (via A1 adenosine receptors) and of 5-HT (via 5-HT1A receptors) were completely additive, indicating that A1 adenosine and 5-HT1A receptors were coupled to distinct pools of G-proteins in hip...Continue Reading

References

Oct 1, 1992·Trends in Biochemical Sciences·J R Hepler, A G Gilman
Jul 1, 1992·Scientific American·M E Linder, A G Gilman
Sep 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J Linden
Jul 1, 1990·Physiological Reviews·R A Olsson, J D Pearson
Dec 1, 1985·The Biochemical Journal·V HausleithnerW Schütz
May 1, 1986·Journal of Neurochemistry·K N KlotzU Schwabe
Feb 1, 1986·Naunyn-Schmiedeberg's Archives of Pharmacology·B B FredholmK Lindström
Feb 15, 1995·European Journal of Pharmacology·Y Odagaki, K Fuxe
Oct 1, 1993·Trends in Pharmacological Sciences·M G Collis, S M Hourani
Mar 1, 1959·British Journal of Pharmacology and Chemotherapy·O ARUNLAKSHANA, H O SCHILD

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Citations

Aug 1, 1997·British Journal of Pharmacology·Y OdagakiT Koyama
May 23, 2014·Journal of Pharmacological Sciences·Yuji OdagakiRyoichi Toyoshima

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