Functional coupling in bovine ciliary epithelial cells is modulated by carbachol

The American Journal of Physiology
J W Stelling, T J Jacob

Abstract

The functional coupling of the ciliary epithelium was studied in isolated pairs (couplets) of pigmented ciliary epithelial (PCE) and nonpigmented ciliary epithelial (NPCE) cells using the whole cell patch clamp and the fluorescent dye lucifer yellow. One cell of the pair (usually the NPCE cell of a NPCE-PCE cell couplet) was accessed with a 2-5 M omega electrode, containing 1-2 mM lucifer yellow, in the whole cell configuration of the patch clamp. After voltage-clamp experiments were completed, cells were viewed under a fluorescent microscope to confirm that the cells were coupled. The electrical coupling of the cells was also studied by calculating the capacitance (using the time-domain technique), assuming a "supercell" model for coupled cells. The mean capacitance of coupled pairs was 79.8 +/- 4.3 (SE) pF (n = 47) compared with single cell capacitances of 36.8 +/- 3.4 pF (n = 10) for PCE cells and 38.1 +/- 3.1 pF (n = 15) for NPCE cells. Octanol, carbachol (CCh), and raised extracellular Ca2+ concentration ([Ca2+]o) all caused uncoupling in pairs (couplets) of coupled NPCE and PCE cells. At room temperature (22-24 degrees C), the capacitance of the couplets decreased from 70.5 +/- 8.0 to 48.0 +/- 5.2 pF (n = 5) when exposed ...Continue Reading

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Citations

Apr 25, 2000·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·L A AlvesW Savino
Sep 24, 2004·The Journal of Membrane Biology·C W Do, M M Civan
Oct 19, 2006·Journal of Cell Science·Mónica R CaleraDaniel A Goodenough
Jul 19, 2002·Experimental Eye Research·Kirsten L CoffeyPaul J Donaldson
Dec 6, 2008·Experimental Eye Research·Chi Wai Do, Mortimer M Civan

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