PMID: 9185160Jul 1, 1997Paper

Functional coupling of Gi subtype with GABAB receptor/adenylyl cyclase system: analysis using a reconstituted system with purified GTP-binding protein from bovine cerebral cortex

Neurochemistry International
M NishikawaK Kuriyama

Abstract

A single molecular species of GTP-binding protein (G protein) has been purified from the bovine cerebral cortex. The immunoblot analysis indicated that the isolated G protein might be Gi1 or Gi2 but not Go, since it was reacted by specific antibodies, anti-Gi alpha 1-2 and anti-Gi alpha 1-1, but not anti-Go alpha. When the Gi protein was reconstituted into phospholipid vesicles with partially purified GABAB receptor and adenylyl cyclase, the stimulation of GABAB receptor by its agonists induced the inhibition of forskolin-stimulated cAMP accumulation. This GABA-induced inhibition was abolished by CGP 55845A, an antagonist of GABAB receptor. These results suggest that a Gi subtype, which was suggested to correspond to Gi1 or Gi2 may be functionally coupled with GABAB receptor/adenylyl cyclase system.

References

Jan 1, 1991·Annual Review of Biochemistry·Y KaziroT Satoh
Oct 15, 1990·Biochemical and Biophysical Research Communications·Y Ohmori, K Kuriyama
Oct 1, 1990·FEBS Letters·R MorishitaT Asano
Mar 1, 1988·Trends in Neurosciences·J Bormann
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Jan 6, 1995·The Journal of Biological Chemistry·R Taussig, A G Gilman
Jul 1, 1993·Trends in Pharmacological Sciences·G Bonanno, M Raiteri

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Citations

May 7, 2005·Neuroscience and Behavioral Physiology·A V Sem'yanov
Jul 22, 1998·Progress in Neurobiology·C L FaingoldA Riaz
Aug 12, 2006·Canadian Journal of Physiology and Pharmacology·Samuel B KombianWandikayi C Matowe
Jan 24, 2006·Pharmacology & Therapeutics·J L Weiner, C F Valenzuela

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