PMID: 8614269Jan 1, 1996Paper

Functional coupling of the Na+/Ca2+ exchanger with Ca2+ release from intracellular stores in cultured smooth muscle cells of guinea pig ileum

Life Sciences
H OhataK Momose

Abstract

The mechanism of increase in intracellular Ca2+ concentration ([Ca2+]i) by removal of extracellular Na+, which phenomena were reported previously (Japan. J. Pharmacol. 63 83-91 1993), was investigated in cultured guinea pig ileum longitudinal muscle cells loaded with a fluorescent Ca2+ indicator, fura-2, by digital ratio imaging microscopy. Isotonic substitution of choline chloride for NaCl induced a transient increase in [Ca2+]i. The pretreatment of thapsigargin (0.5 microM), but not nicardipine (10 microM), suppressed the transient increase completely. In solutions containing micromolar concentrations of free Ca2+ (nominally Ca2+-free solution), the Na+-free induced transient increase was observed, but neither the second cell exposure to the Na+-free solution nor the following application of histamine increased [Ca2+]i, indicating that removal of extracellular Na+ releases Ca2+ from intracellular stores including inositol 1,4,5-trisphosphate (IP3)-releasable pools. The Na+-free induced transient increase required the presence of more than micromolar concentrations of extracellular free Ca2+ and releasable Ca2+ within the stores, but ryanodine did not affect the transient increase. These results suggest that undetectable influ...Continue Reading

Citations

Oct 2, 1998·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Y FangC Zhou
Jan 28, 1999·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Y FangL He
Aug 1, 1997·British Journal of Pharmacology·J CortijoR C Small
Apr 28, 2006·Pediatric Research·Omar ParvezJaques Belik
May 17, 2002·Research in Nursing & Health·Inger S JohanssonGerry Larsson
Aug 3, 2000·American Journal of Physiology. Heart and Circulatory Physiology·A ArnonM P Blaustein

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