Functional cross-talk between angiotensin II and epidermal growth factor receptors in NIH3T3 fibroblasts

Journal of Hypertension
Paola De PaolisMassimo Volpe

Abstract

The main angiotensin (Ang) II subtype receptors (AT1R and AT2R) are involved in cellular growth processes and exert functionally antagonistic effects. To characterize the mechanisms by which Ang II receptors influence growth, by investigating the interactions between Ang II subtype receptors and epidermal growth factor (EGF) receptors on mitogen-activated protein kinase (MAPK) pathway activation. The experiments were performed using a mouse fibroblast cell line, NIH3T3, by transient co-transfection with rat AT1R or AT2R expression vectors, or both. Extracellular-signal-regulated kinase (ERK)1/2 phosphorylation was analysed by western blot and the ERK activity was evaluated using PathDetect, an in-vivo signal transduction pathway trans-reporting system. Selective Ang II receptor antagonists (losartan for AT1R and PD123319 for AT2R) were used to investigate the contributions of each receptor to the response observed. Our data show that, in this cellular model, both Ang II receptors phosphorylate ERK1/2. However, in the cells expressing AT1R, the EGF-induced MAPK pathway was enhanced in the presence of Ang II in a synergistic fashion. In contrast, a reduction of EGF-induced MAPK activation was observed in the cells expressing AT2R...Continue Reading

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Citations

Mar 23, 2002·Journal of Hypertension·Joshua S Newton
Dec 18, 2003·Clinical and Experimental Pharmacology & Physiology·Yi-Chun ZhuTai Yao
Apr 10, 2009·The Journal of Pharmacology and Experimental Therapeutics·Xiaoping ZhangGuangyu Wu
Oct 29, 2004·The American Journal of Pathology·Hikaru TakedaShigeo Kondo
Sep 12, 2014·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Isha DhandeTahir Hussain
Oct 29, 2009·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Catherine A Lemarié, Ernesto L Schiffrin
May 5, 2004·The Journal of Biological Chemistry·Sandrine NouetClara Nahmias
Apr 9, 2019·Acta Physiologica·Colin SumnersUlrike Muscha Steckelings
Jul 23, 2003·Journal of Hypertension·Massimo VolpeAlberto Morganti
Dec 6, 2002·Journal of the American Society of Nephrology : JASN·Massimo VolpeSperanza Rubattu

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