PMID: 6160040Oct 1, 1980Paper

Functional desensitisation of beta-adrenergic receptors of avian erythrocytes by catecholamines and adenosine 3',5'-phosphate

European Journal of Biochemistry
I A Simpson, T Pfeuffer

Abstract

Prolonged exposure to beta-adrenergic agonists of pigeon erythrocytes causes a reversible loss (70%) of catecholamine-stimulated adenylate cyclase activity without reduction in the number of beta-adrenergic receptors. In addition a less pronounced decrease in non-stimulated and NaF-stimulated adenylate cyclase activity (15-22%) is observed, appearing at different agonist concentrations and at a different rate. Dibutyryladenosine 3',5'-phosphate and the phosphodiesterase inhibitor methylisobutylxanthine partially mimick the action of the beta-adrenergic agonist, thus pointing to a possible role of adenosine 3',5'-phosphate in establishing desensitization. When adenylate cyclase from desensitized cells is stimulated with 5'-guanylyl-imidodiphosphate in the presence or absence of catecholamines the lag period preceding the attainment of maximal activity is extended. Likewise the rate of reversal by GTP or GTP of persistent activation of adenylate cyclase is slowed down. This is therefore interpreted to mean that the loss in hormonal stimulation on treatment of pigeon red blood cells with beta-adrenergic agonists is due to a delayed exchange of GDP against GTP on the regulatory GTP-binding protein. Furthermore, we conclude that eve...Continue Reading

References

Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D S NewcombeJ V Fahey
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D Cassel, T Pfeuffer
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D M Chuang, E Costa
May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·C MukherjeeR J Lefkowitz
Apr 1, 1974·Analytical Biochemistry·Y SalomonM Rodbell

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Citations

May 1, 1987·Pflügers Archiv : European journal of physiology·E Kumamoto, K Kuba
Jan 1, 1985·The Journal of Membrane Biology·R J LefkowitzM G Caron
Jan 1, 1987·Molecular Neurobiology·D R Sibley, R J Lefkowitz
Jan 23, 1999·European Journal of Biochemistry·P FerreiraT Pfeuffer
Jan 1, 1985·Pharmacology & Therapeutics·J M Stadel

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