PMID: 11897839Mar 19, 2002Paper

Functional differences between alpha subunit isoforms of the rat Na,K-ATPase expressed in Xenopus oocytes

The Journal of Physiology
Jean-Daniel Horisberger, Solange Kharoubi-Hess

Abstract

The functional properties of the three most widely distributed alpha subunit isoforms of the Na,K-ATPase are not well known, particularly concerning the voltage dependence of their activity and cation binding kinetics. We measured the electrogenic activity generated by Na,K-ATPases resulting from co-expression of the rat alpha1, alpha2* or alpha3* subunits with the rat beta1 subunit in Xenopus oocytes; alpha2* and alpha3* are ouabain-resistant mutants of the alpha2 and alpha3 isoform, which allowed selective inhibition of the endogenous Na(+),K(+)-pump of the oocyte. In oocytes expressing the three isoforms of the alpha subunit, K(+) induced robust outward currents that were largely ouabain-sensitive. In addition, ouabain-sensitive inward currents were recorded for all three isoforms in sodium-free and potassium-free acid solutions. The very similar voltage dependence of the Na(+),K(+)-pump activity observed in the absence of extracellular Na(+) indicated a similar stoichiometry of the transported cations by the three isoforms. The affinity for extracellular K(+) was slightly lower for the alpha2* and alpha3* than for the alpha1 isoform. The alpha2* isoform was, however, more sensitive to voltage-dependent inhibition by extrace...Continue Reading

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Citations

Sep 8, 2012·Cellular and Molecular Life Sciences : CMLS·W FullerK J Wypijewski
Mar 10, 2007·The Journal of Membrane Biology·Ciming LiJean-Daniel Horisberger
Aug 11, 2010·The Journal of Membrane Biology·Yanli Ding, Robert F Rakowski
Aug 31, 2010·The Journal of Biological Chemistry·Michael J MortonMichael J Caplan
Apr 27, 2005·The Journal of General Physiology·Robert E DempskiErnst Bamberg
Mar 1, 2006·The Journal of General Physiology·Oihana CapendeguyJean-Daniel Horisberger
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May 16, 2013·Pflügers Archiv : European journal of physiology·Alfonso Bueno-OrovioBlanca Rodriguez
Aug 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ciming LiJean-Daniel Horisberger
Mar 19, 2005·The Journal of Physiology·Oihana Capendeguy, Jean-Daniel Horisberger
May 13, 2006·The Journal of Physiology·Arun AnantharamLawrence G Palmer
Mar 14, 2007·Biochemical and Biophysical Research Communications·Rossen RadkovJean-Daniel Horisberger
May 2, 2003·British Journal of Pharmacology·Kanako HamadaHitoshi Yasuda
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Jan 22, 2021·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Katherine Medina-OrtizSantiago Castaño

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