Functional differences between two morphologically distinct cell subpopulations within a human colorectal carcinoma cell line

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
A C SolimeneJ D Lopes

Abstract

The LISP-I human colorectal adenocarcinoma cell line was isolated from a hepatic metastasis at the Ludwig Institute, São Paulo, SP, Brazil. The objective of the present study was to isolate morphologically different subpopulations within the LISP-I cell line, and characterize some of their behavioral aspects such as adhesion to and migration towards extracellular matrix components, expression of intercellular adhesion molecules and tumorigenicity in vitro. Once isolated, the subpopulations were submitted to adhesion and migration assays on laminin and fibronectin (crucial proteins to invasion and metastasis), as well as to anchorage-independent growth. Two morphologically different subpopulations were isolated: LISP-A10 and LISP-E11. LISP-A10 presents a differentiated epithelial pattern, and LISP-E11 is fibroblastoid, suggesting a poorly differentiated pattern. LISP-A10 expressed the two intercellular adhesion molecules tested, carcinoembryonic antigen (CEA) and desmoglein, while LISP-E11 expressed only low amounts of CEA. On the other hand, adhesion to laminin and fibronectin as well as migration towards these extracellular matrix proteins were higher in LISP-E11, as expected from its poorly differentiated phenotype. Both subp...Continue Reading

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Citations

Sep 1, 2005·Cellular and Molecular Neurobiology·André A SchenkaJosé Vassallo
May 20, 2006·International Journal of Experimental Pathology·Luciana Doria GuarinielloCélia Regina Whitaker Carneiro
Jul 30, 2003·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·R A RoelaM H H Federico
Oct 17, 2008·Bulletin of Experimental Biology and Medicine·O V SeminaA S Saenko
Dec 9, 2010·Arquivos De Gastroenterologia·Denise Gonçalves PriolliJaques Waisberg
Dec 4, 2016·Cancer Immunology, Immunotherapy : CII·Priscila M A DenapoliSang W Han
Jul 19, 2006·International Journal of Biological Macromolecules·Adriana M NakahataMaria Luiza V Oliva

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