Functional differences in visceral and subcutaneous fat pads originate from differences in the adipose stem cell.

PloS One
Silvana BaglioniMichaela Luconi

Abstract

Metabolic pathologies mainly originate from adipose tissue (AT) dysfunctions. AT differences are associated with fat-depot anatomic distribution in subcutaneous (SAT) and visceral omental (VAT) pads. We address the question whether the functional differences between the two compartments may be present early in the adipose stem cell (ASC) instead of being restricted to the mature adipocytes. Using a specific human ASC model, we evaluated proliferation/differentiation of ASC from abdominal SAT-(S-ASC) and VAT-(V-ASC) paired biopsies in parallel as well as the electrophysiological properties and functional activity of ASC and their in vitro-derived adipocytes. A dramatic difference in proliferation and adipogenic potential was observed between the two ASC populations, S-ASC having a growth rate and adipogenic potential significantly higher than V-ASC and giving rise to more functional and better organized adipocytes. To our knowledge, this is the first comprehensive electrophysiological analysis of ASC and derived-adipocytes, showing electrophysiological properties, such as membrane potential, capacitance and K(+)-current parameters which confirm the better functionality of S-ASC and their derived adipocytes. We document the great...Continue Reading

References

Feb 17, 2000·The Journal of Clinical Investigation·O GavrilovaM L Reitman
Apr 18, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Tamara TchkoniaJames L Kirkland
Jun 26, 2003·Trends in Pharmacological Sciences·Max Lafontan, Michel Berlan
Oct 28, 2003·The Journal of Physiology·Jürgen F HeubachUrsula Ravens
Jan 16, 2004·The Journal of Membrane Biology·M P Ramírez-PonceJ A Bellido
Feb 10, 2004·Biochemical and Biophysical Research Communications·Anne-Marie RodriguezGérard Ailhaud
Mar 9, 2004·Biochemical and Biophysical Research Communications·Halima Ouadid-AhidouchNatalia Prevarskaya
Jun 3, 2004·The Journal of Clinical Investigation·Soren NielsenMichael D Jensen
Sep 24, 2004·American Journal of Physiology. Endocrinology and Metabolism·Tamara TchkoniaJames L Kirkland
Mar 1, 2005·Biochimie·A-M RodriguezC Dani
Apr 9, 2005·The American Journal of Clinical Nutrition·Zimian WangDympna Gallagher
Apr 19, 2005·Cancer Research·Daniel RubioAntonio Bernad
Jun 1, 2005·Experimental Cell Research·Andrea DickerMikael Rydén
Jul 16, 2005·Journal of Cellular Physiology·Farshid GuilakJeffrey M Gimble
Mar 29, 2006·Diabetes·Luigi LaviolaFrancesco Giorgino
Sep 21, 2006·American Journal of Physiology. Endocrinology and Metabolism·Tamara TchkoniaJames L Kirkland
Feb 9, 2008·Stem Cells·Coralie FontainePascal Peraldi
May 8, 2008·Cell Metabolism·Thien T TranC Ronald Kahn
Sep 26, 2008·PLoS Biology·Samuel Virtue, Antonio Vidal-Puig
Oct 23, 2008·Journal of Cellular Physiology·Hao HuGui-Rong Li
Nov 14, 2008·The New England Journal of Medicine·T PischonE Riboli
Mar 10, 2009·Genes & Development·Christopher A FasanoSally Temple
Jun 30, 2009·Stem Cell Reviews and Reports·Sarah SundelacruzDavid L Kaplan
Jul 9, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Silvana BaglioniMichaela Luconi

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Citations

May 3, 2014·PloS One·Hyung Sun YooJianhua Shao
Aug 15, 2014·PloS One·Mayuri PrasadCristian Pablo Pennisi
Jan 7, 2015·Regenerative Medicine·Kavan S JohalAdam J Reid
Aug 19, 2015·Nature Cell Biology·Qiong A WangPhilipp E Scherer
Jun 12, 2013·Biochimica Et Biophysica Acta·Joan Sanchez-Gurmaches, David A Guertin
Jul 15, 2015·Adipocyte·Nicole C SmithSherri L Christian
Aug 12, 2014·Human Reproduction·Héctor F Escobar-MorrealeManuel Luque-Ramírez
May 24, 2013·Adipocyte·Michelle T Foster, Michael J Pagliassotti
Jan 30, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Poli Mara SpritzerDebora M Morsch
Jan 13, 2015·Molecular and Cellular Endocrinology·Giulia CantiniMichaela Luconi
Jul 28, 2016·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Boyun KimYong Sang Song
Apr 29, 2016·American Journal of Human Biology : the Official Journal of the Human Biology Council·Jingjing SunJeanne Freeland-Graves
Jun 21, 2016·Cell Metabolism·Elise JefferyMatthew S Rodeheffer
May 27, 2017·Cardiovascular Research·Alexios S Antonopoulos, Dimitris Tousoulis
Mar 9, 2018·The Journal of Experimental Biology·Panna TandonJames E N Minchin
Jul 12, 2018·Chronobiology International·Maureen FriedrichsHenrik Oster
Aug 10, 2013·Circulation. Cardiovascular Imaging·Ian J NeelandMark H Drazner
Mar 8, 2018·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Emil AndersenRomain Barrès
Mar 9, 2019·Adipocyte·Nida Haider, Louise Larose
Jul 6, 2019·Obesity Surgery·Adriana Florinela Cătoi, Luca Busetto
Sep 9, 2019·Endocrine·Rosiane Aparecida MirandaPatrícia C Lisboa
Aug 30, 2019·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Sebastio PerriniFrancesco Giorgino
Dec 24, 2019·Annals of the New York Academy of Sciences·John R BrachtDeborah J Clegg
Nov 20, 2016·Journal of Cellular and Molecular Medicine·Kimberly A Cox-YorkRachael E Van Pelt

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Methods Mentioned

BETA
biopsies
ELISA
flow cytometry
fluorescence microscopy
dissection

Software Mentioned

CellQuest
Chantal
pClamp9
Scepter
FACScan
SPSS

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