PMID: 9435087Jan 22, 1998Paper

Functional display of a heterologous protein on the surface of Lactococcus lactis by means of the cell wall anchor of Staphylococcus aureus protein A

Applied and Environmental Microbiology
L SteidlerE Remaut

Abstract

In this study, we showed that the cell wall anchor of protein A from Staphylococcus aureus is functional in the food-grade organism Lactococcus lactis. A fusion protein composed of the lactococcal Usp45 secretion signal peptide, streptavidin monomer, and the S. aureus protein A anchor became covalently attached to the peptidoglycan when expressed in L. lactis. The streptavidin moiety of the fusion protein was functionally exposed at the cellular surface. L. lactis cells expressing the anchored fusion polypeptide could be specifically immobilized on a biotinylated alkaline phosphatase-coated polystyrene support.

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Citations

Jan 11, 2003·Biotechnology & Genetic Engineering Reviews·Walter MaglianiLuciano Polonelli
May 26, 2010·Journal of Applied Microbiology·K Kylä-NikkiläP E J Saris
Sep 5, 2002·Biotechnology and Bioengineering·Wilfred Chen, George Georgiou
Sep 21, 2010·Applied and Environmental Microbiology·Yakhya DieyeJean-Christophe Piard
Dec 8, 2007·Microbiology and Molecular Biology Reviews : MMBR·Joachim Jose, Thomas F Meyer
Mar 10, 2015·Applied Microbiology and Biotechnology·Agnieszka WyszyńskaElżbieta Katarzyna Jagusztyn-Krynicka
Sep 13, 2011·Microbial Biotechnology·Anika C Jahns, Bernd H A Rehm
Jun 29, 1999·Current Opinion in Microbiology·W M de Vos
Jun 1, 2002·Journal of Biotechnology·Patrik SamuelsonStefan Ståhl
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·W W Navarre, O Schneewind
May 17, 2005·Insect Biochemistry and Molecular Biology·Michael A Riehle, Marcelo Jacobs-Lorena

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