Functional disruption of the prion protein gene in cloned goats

The Journal of General Virology
Guohua YuGuoxiang Cheng

Abstract

The cellular prion protein (PrPC), a membrane glycoprotein anchored to the outer surface of neurons, lymphocytes and other cells, is associated directly with the pathogenesis of the transmissible spongiform encephalopathies (TSEs) occurring mainly in humans, cattle, sheep and goats. Although mice lacking PrPC develop and reproduce normally and are resistant to scrapie infection, large animals lacking PrPC, especially those species in which TSE occurs naturally, are currently not available. Here, five live PRNP+/- goats cloned by gene targeting are reported. Detailed RNA-transcription and protein-expression analysis of one PRNP+/- goat showed that one allele of the caprine PRNP gene had been disrupted functionally. No gross abnormal development or behaviour could be seen in these PRNP+/- goats up to at least 3 months of age. These heterozygous PRNP+/- goats are ready to be used in producing homozygous PRNP-/- goats in which no PrPC should be expressed.

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Citations

Sep 30, 2008·Transgenic Research·Caihong ZhuGuoxiang Cheng
Apr 25, 2012·Transgenic Research·Laurent BoulangerJean-Luc Vilotte
Feb 6, 2009·Applied Biochemistry and Biotechnology·Xueming ZhangBoyang Yu
Nov 9, 2010·Molecular Biotechnology·Claudia MerklAngelika Schnieke
May 20, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Christopher S RogersMichael J Welsh
Mar 28, 2008·BMC Biotechnology·Daniel T MeehanRobert B Norgren
May 10, 2012·PLoS Pathogens·Adriano Aguzzi, Caihong Zhu
Dec 4, 2012·Journal of Biotechnology·Shaohua WangNing Li
Feb 5, 2008·Comparative Immunology, Microbiology and Infectious Diseases·G Laible
Jul 17, 2009·Biotechnology Journal·Götz Laible, Lucía Alonso-González
Apr 9, 2016·Expert Reviews in Molecular Medicine·Paula SaáLarisa Cervenakova
Feb 1, 2015·Cellular and Molecular Life Sciences : CMLS·Pablo BoschWilfried A Kues
Sep 5, 2014·PloS One·Wei NiChuangfu Chen
Jun 18, 2017·The Journal of Gene Medicine·Puping LiangJunjiu Huang
Oct 28, 2008·Molecular Reproduction and Development·Guohua YuGuoxiang Cheng
Apr 1, 2007·Prion·Andrew D SteeleAdriano Aguzzi
May 4, 2017·BMC Biology·Marie-Angela WulfAdriano Aguzzi
Jul 18, 2017·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Bhanu P TeluguChi-Hun Park
Dec 6, 2018·Nature Reviews. Genetics·Michel GeorgesBen Hayes
Jul 16, 2019·NPJ Science of Food·Alison L Van EenennaamJames D Murray
Jan 29, 2016·Transgenic Research·L R BertoliniJ D Murray
Sep 26, 2019·Frontiers in Genetics·Peter KaldsYulin Chen
Nov 10, 2017·Emerging Topics in Life Sciences·Chris Proudfoot, Christine Burkard

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