Functional dissection of a cell-division inhibitor, SulA, of Escherichia coli and its negative regulation by Lon

Molecular & General Genetics : MGG
A HigashitaniK Koriuchi

Abstract

SulA is induced in Escherichia coli by the SOS response and inhibits cell division through interaction with FtsZ. To determine which region of SulA is essential for the inhibition of cell division, we constructed a series of N-terminal and C-terminal deletions of SulA and a series of alanine substitution mutants. Arginine at position 62, leucine at 67, tryptophan at 77 and lysine at 87, in the central region of SulA, were all essential for the inhibitory activity. Residues 3-27 and the C-terminal 21 residues were dispensable for the activity. The mutant protein lacking N-terminal residues 3-47 was inactive, as was that lacking the C-terminal 34 residues. C-terminal deletions of 8 and 21 residues increased the growth-inhibiting activity in lon+ cells, but not in lon- cells. The wild-type and mutant SulA proteins were isolated in a form fused to E. coli maltose-binding protein, and tested in vitro for sensitivity to Lon protease. Lon degraded wild-type SulA and a deletion mutant lacking the N-terminal 93 amino acids, but did not degrade the derivative lacking 21 residues at the C-terminus. Furthermore, the wild-type SulA and the N-terminal deletion mutant formed a stable complex with Lon, while the C-terminal deletion did not. MB...Continue Reading

Citations

Sep 25, 2007·Archives of Microbiology·Shen LuoRodney L Levine
Aug 31, 2012·Cell Biochemistry and Biophysics·Weiling HongJianping Xie
Mar 24, 2004·Journal of Structural Biology·Ae-Ran KwonDavid B McKay
Feb 23, 2002·Trends in Cell Biology·Jean Ju ChungMin Li
Feb 22, 2002·European Journal of Biochemistry·Wataru NishiiKenji Takahashi
Jul 15, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·K IshiokaH Shinagawa
Jan 30, 2013·Protein Engineering, Design & Selection : PEDS·Matthew L WohleverRobert T Sauer
Nov 10, 2013·Genome Announcements·Andrey V Karlyshev, Vyacheslav G Melnikov
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Yu-Ling Shih, Lawrence Rothfield
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·H K SongR Huber
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ravishankar RamachandranMatthias Bochtler
Apr 2, 2005·Protein Science : a Publication of the Protein Society·M Kamran AzimPeter Goettig
Jan 22, 2005·Molecular Microbiology·Liliana C Losada, Steven W Hutcheson
Apr 25, 2006·Molecular Microbiology·Susan M ButlerK Heran Darwin
Oct 1, 2010·Environmental Microbiology Reports·Akiko HamamotoAkira Takahashi
Jan 21, 2010·Biophysical Journal·Jiahn-Haur LiaoShih-Hsiung Wu
Oct 7, 2003·Annual Review of Microbiology·Laura Romberg, Petra Anne Levin
May 24, 2007·Journal of Basic Microbiology·Ramanujam Srinivasan, Parthasarathi Ajitkumar
Jul 3, 2013·The Journal of Biological Chemistry·Kwang Hoon SungHyun Kyu Song
Oct 3, 2020·Acta Crystallographica. Section F, Structural Biology Communications·Rahul SinghRavindra D Makde

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