PMID: 9177203Jun 10, 1997Paper

Functional dissection of the Drosophila enhancer of split protein, a suppressor of neurogenesis

Proceedings of the National Academy of Sciences of the United States of America
B Giebel, J A Campos-Ortega

Abstract

The Enhancer of split [E(spl)] gene complex of Drosophila comprises seven related genes encoding a special type of basic helix-loop-helix proteins, the function of which is to suppress the neural developmental fate. One of these proteins is E(spl) itself. To gain insight into the structural requirements for E(spl) function, we have expressed a large number of deletion variants in transgenic flies. Three protein domains were identified as essential for suppression of bristle development: the carboxyl-terminal tetrapeptide WRPW, the region comprising the putative helix III and helix IV, and the region between helix IV and the WRPW motif. Lack of the basic helix-loop-helix domain, helix III or IV, only partially inhibits the suppressor activity of the protein. Truncated variants that lack all the regions carboxyl-terminal to helix IV elicit the development of additional neural progenitors, and thus act as dominant-negative variants. All these results suggest that E(spl) suppresses neural development by direct interaction with other proteins, such as groucho and the proneural proteins.

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Citations

Feb 23, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·N E Baker
Nov 26, 2002·EMBO Reports·Anne-Isabelle MunierMarie Lagueux
Dec 11, 2003·Genes & Development·Hamed Jafar-NejadHugo J Bellen
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Jun 12, 2014·Applied Biochemistry and Biotechnology·Min LiuZuoming Nie
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Jun 19, 2009·Genesis : the Journal of Genetics and Development·Ezgi Kunttas-TatliAshok P Bidwai
May 6, 2009·Genesis : the Journal of Genetics and Development·Bhaskar KahaliAshok P Bidwai
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Dec 17, 2009·Genesis : the Journal of Genetics and Development·Bhaskar KahaliAshok P Bidwai
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Aug 26, 2006·Mechanisms of Development·Anasua BoseAshok P Bidwai
Feb 1, 2003·Mechanisms of Development·Nicolas NègreAnne Marie Martinez
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Dec 6, 2002·Development·Nikolaos GiagtzoglouChristos Delidakis
Jun 1, 2004·Development·Stefan HansJosé A Campos-Ortega

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