Functional diversification of Arabidopsis SEC1-related SM proteins in cytokinetic and secretory membrane fusion

Proceedings of the National Academy of Sciences of the United States of America
Matthias KarnahlGerd Jürgens

Abstract

Sec1/Munc18 (SM) proteins contribute to membrane fusion by interacting with Qa-SNAREs or nascent trans-SNARE complexes. Gymnosperms and the basal angiosperm Amborella have only a single SEC1 gene related to the KEULE gene in Arabidopsis However, the genomes of most angiosperms including Arabidopsis encode three SEC1-related SM proteins of which only KEULE has been functionally characterized as interacting with the cytokinesis-specific Qa-SNARE KNOLLE during cell-plate formation. Here we analyze the closest paralog of KEULE named SEC1B. In contrast to the cytokinesis defects of keule mutants, sec1b mutants are homozygous viable. However, the keule sec1b double mutant was nearly gametophytically lethal, displaying collapsed pollen grains, which suggests substantial overlap between SEC1B and KEULE functions in secretion-dependent growth. SEC1B had a strong preference for interaction with the evolutionarily ancient Qa-SNARE SYP132 involved in secretion and cytokinesis, whereas KEULE interacted with both KNOLLE and SYP132. This differential interaction with Qa-SNAREs is likely conferred by domains 1 and 2a of the two SM proteins. Comparative analysis of all four possible combinations of the relevant SEC1 Qa-SNARE double mutants reve...Continue Reading

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Citations

Nov 19, 2018·Biophysics Reviews·Kenneth S Gerien, Jian-Qiu Wu
Dec 26, 2018·Frontiers in Plant Science·Rosalie SinclairGeorgia Drakakaki
Feb 20, 2021·Annual Review of Plant Biology·Thomas Dresselhaus, Gerd Jürgens
Sep 16, 2021·The Plant Cell·Alice Y CheungDolf Weijers

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