Functional Diversification of Replication Protein A Paralogs and Telomere Length Maintenance in Arabidopsis.

Genetics
Behailu B AkliluDorothy E Shippen

Abstract

Replication protein A (RPA) is essential for many facets of DNA metabolism. The RPA gene family expanded in Arabidopsis thaliana with five phylogenetically distinct RPA1 subunits (RPA1A-E), two RPA2 (RPA2A and B), and two RPA3 (RPA3A and B). RPA1 paralogs exhibit partial redundancy and functional specialization in DNA replication (RPA1B and RPA1D), repair (RPA1C and RPA1E), and meiotic recombination (RPA1A and RPA1C). Here, we show that RPA subunits also differentially impact telomere length set point. Loss of RPA1 resets bulk telomeres at a shorter length, with a functional hierarchy for replication group over repair and meiosis group RPA1 subunits. Plants lacking RPA2A, but not RPA2B, harbor short telomeres similar to the replication group. Telomere shortening does not correlate with decreased telomerase activity or deprotection of chromosome ends in rpa mutants. However, in vitro assays show that RPA1B2A3B unfolds telomeric G-quadruplexes known to inhibit replications fork progression. We also found that ATR deficiency can partially rescue short telomeres in rpa2a mutants, although plants exhibit defects in growth and development. Unexpectedly, the telomere shortening phenotype of rpa2a mutants is completely abolished in pla...Continue Reading

References

Apr 25, 1991·Nature·A M ZahlerD M Prescott
Jun 10, 1995·Molecular & General Genetics : MGG·J FajkusM Bezdĕk
Jun 1, 1996·Genes & Development·B Li, A J Lustig
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·M S FitzgeraldD E Shippen
Dec 10, 1999·The Plant Cell·P J KrysanM R Sussman
Feb 19, 2002·Oncogene·Jeremy D HensonRoger R Reddel
Jan 1, 2004·Nature Genetics·Vera SchramkeVincent Géli
Jul 20, 2004·The Plant Cell·Eugene V Shakirov, Dorothy E Shippen
Jul 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Arthur J ZaugThomas R Cech
Sep 16, 2006·Nucleic Acids Research·Tonatiuh Romero SalasCarole Saintomé
Feb 13, 2007·Nature Structural & Molecular Biology·Hua GaoVictoria Lundblad
Apr 13, 2007·Molecular Biology of the Cell·Tatsuya KibeMasaru Ueno
Jan 24, 2008·Molecular and Cellular Biology·Kalpana KannanDorothy E Shippen
Oct 30, 2008·Cell·Louise J BarberSimon J Boulton
Dec 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Xiangyu SongDorothy E Shippen
Jan 23, 2009·The EMBO Journal·Anja IrmischJohanne M Murray
Jan 26, 2010·DNA Repair·Victoria Marini, Lumir Krejci
Feb 9, 2010·Bioscience, Biotechnology, and Biochemistry·Yuka KobayashiMasaru Ueno
Jul 20, 2010·FEBS Letters·Katrin PaeschkeVirginia A Zakian
Nov 5, 2010·Annual Review of Genetics·Devanshi Jain, Julia Promisel Cooper
Nov 16, 2010·Nature Structural & Molecular Biology·Sophie BadieMadalena Tarsounas
Dec 24, 2010·Nucleic Acids Research·Phong Lan Thao TranPatrizia Alberti
Feb 23, 2012·The EMBO Journal·Pierre LucianoVincent Géli
Mar 1, 2012·Cell Cycle·Rachel Litman FlynnLee Zou
Mar 7, 2012·Experimental Cell Research·Virginia A Zakian
Nov 12, 2013·Molecular Cell·Shunping YanXinnian Dong
May 13, 2014·DNA Repair·Ana María León-OrtizSimon J Boulton
Sep 7, 2014·Experimental Cell Research·Maartje van Kregten, Marcel Tijsterman

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Citations

May 29, 2021·Planta·Supriyo ChowdhurySupriya Chakraborty

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