Functional diversification of the potato R2R3 MYB anthocyanin activators AN1, MYBA1, and MYB113 and their interaction with basic helix-loop-helix cofactors

Journal of Experimental Botany
Yuhui LiuAndrew C Allan

Abstract

In potato (Solanum tuberosum L.), R2R3 MYBs are involved in the regulation of anthocyanin biosynthesis. We examined sequences of these MYBs in cultivated potatoes, which are more complex than diploid potato due to ploidy and heterozygosity. We found amino acid variants in the C-terminus of the MYB StAN1, termed R0, R1, and R3, due to the presence of a repeated 10-amino acid motif. These variant MYBs showed some expression in both white and pigmented tubers. We found several new alleles or gene family members of R2R3 MYBs,StMYBA1 and StMYB113, which were also expressed in white potato tubers. From functional analysis in tobacco, we showed that the presence of a C-terminal 10-amino acid motif is optimal for activating anthocyanin accumulation. Engineering a motif back into a MYB lacking this sequence enhanced its activating ability. Versions of StMYBA1 and StMYB113 can also activate anthocyanin accumulation in tobacco leaves, with the exception of StMYB113-3, which has a partial R2R3 domain. We isolated five family members of potato StbHLH1, and one StJAF13, to test their ability to interact with MYB variants. The results showed that two alleles of StbHLH1 from white skin and red skin are non-functional, while three other StbHLH1...Continue Reading

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Citations

Jul 1, 2016·Plant Physiology and Biochemistry : PPB·Kang WeiHao Cheng
Jun 8, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shaoli WangXinhua Ding
May 31, 2019·Journal of Experimental Botany·Varsha TirumalaiPadubidri V Shivaprasad
Apr 26, 2019·Journal of Experimental Botany·Yuhui LiuAndrew C Allan
Nov 1, 2017·BMC Plant Biology·Adolfo Luís Dos SantosVagner Augusto Benedito
Nov 7, 2019·International Journal of Molecular Sciences·Muhammad AnwarLihui Zeng
Mar 20, 2019·BMC Genetics·Ksenia V StryginaElena K Khlestkina
Mar 31, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Muhammad AnwarLihui Zeng
Jan 9, 2019·Frontiers in Plant Science·Nicola BonarCsaba Hornyik
Aug 29, 2020·G3 : Genes - Genomes - Genetics·F Parker E LaimbeerRichard E Veilleux
Nov 8, 2020·Journal of Experimental Botany·Jian-Ping AnYu-Jin Hao
Jan 22, 2020·International Journal of Biological Macromolecules·Yuanming LiYuhui Liu
Mar 23, 2021·Journal of Agricultural and Food Chemistry·Yanfei LiuFengwang Ma
Apr 30, 2021·PloS One·Maria Angelica Parra-GalindoFederico Roda
Apr 27, 2021·Frontiers in Plant Science·Sarita Muñoz-GómezNatalia Pabón-Mora
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hanjo HellmannDuroy A Navarre
Jul 21, 2021·International Journal of Molecular Sciences·Yaqi HaoAigen Fu
Oct 16, 2020·Journal of Agricultural and Food Chemistry·Boyan TangGuoping Chen

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