PMID: 2109912May 1, 1990Paper

Functional domains of the HIV-1 rev gene required for trans-regulation and subcellular localization

Virology
L K VenkateshG Chinnadurai

Abstract

The rev gene of human immunodeficiency virus type 1 (HIV-1) encodes a 116 amino acid nuclear regulatory protein (Rev) that increases the cytoplasmic expression of viral mRNAs containing the Rev response element (RRE) and coding for the structural proteins, Gag and Env. To identify the functional domains of Rev, amino acid deletion and chain termination mutations were introduced in the Rev coding region. The ability of these mutants to increase the cytoplasmic expression of a Rev-test plasmid (pSV-AR), containing the RRE cloned into the 3' noncoding region of the CAT gene in plasmid pSV2CAT, was examined in transient expression assays in HeLa cells. Our results indicate that three distinct regions mapping within the N-terminal 98 amino acids of Rev are essential for its activity. The subcellular localization of the various Rev proteins was examined in COS cells by indirect immunofluorescence. Rev was found to localize predominantly in the nucleolus of transfected cells. All mutant Rev proteins, with the exception of a deletion mutant (rev delta 41-44) lacking four Arg residues of a highly basic domain, were found to localize in the nucleolus. Mutant rev delta 41-44 exhibited weak diffuse fluorescence in the nucleus with a tenden...Continue Reading

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Citations

Dec 4, 2003·Experimental Cell Research·Séverine DemartRuth Brack-Werner
Jan 17, 2007·Nature Reviews. Microbiology·Julian A Hiscox
Jul 19, 2005·Gene·John A DangerfieldWalter H Günzburg
Dec 12, 2003·The Journal of Biological Chemistry·Armelle RoisinPierre Jalinot
Aug 6, 2004·The Journal of Biological Chemistry·Dirk DaelemansGeorge N Pavlakis
Mar 30, 2001·The Journal of Biological Chemistry·D J Goodwin, A Whitehouse
Feb 1, 1996·Biological Chemistry Hoppe-Seyler·Y M HenskensA V Nieuw Amerongen
Jan 1, 1997·Molecular and Cellular Biology·R K ErnstM L Hammarskjöld
Apr 1, 1992·Journal of Virology·M S HorwitzA J Faras
Jun 1, 1991·Microbiological Reviews·K Steffy, F Wong-Staal
Jan 4, 2001·European Journal of Cell Biology·S ThébaultJ M Mesnard

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