Functional effects of antibodies against non-neuronal nicotinic acetylcholine receptors

Immunology Letters
Olena LykhmusMaryna V Skok

Abstract

Non-neuronal nicotinic acetylcholine receptors (nAChRs) are expressed in the spleen and regulate B lymphocyte propagation and activation. The aim of the present study was to investigate the cellular and physiological effects of antibodies against alpha4(1-209) and alpha7(1-208) nAChR extracellular domains. The antibodies, added in vitro, produced in vivo or injected, specifically bound mouse spleen B lymphocytes. Immunization with nAChR extracellular domains resulted in connective tissue overgrowth and infiltration of segmented neutrophils in the spleen, as well as in decreased body weight compared to mice immunized with BSA. In spite of certain cross-reactivity of alpha4(1-209)- and alpha7(1-208)-specific antibodies, all observed effects were more pronounced upon immunization with alpha7 extracellular domain. Spleens of mice injected with alpha7(1-208)-specific antibody contained decreased numbers of Annexin V-positive B lymphocytes compared to mice injected with non-specific IgG. It is concluded that alpha7 nAChRs are involved in regulating the lymphocyte survival, neutrophil migration, connective tissue overgrowth and body weight accumulation. The antibody binding triggers alpha7 nAChR signaling supporting the idea of non-ch...Continue Reading

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Citations

Jan 15, 2014·The International Journal of Biochemistry & Cell Biology·Galyna GergalovaMaryna Skok
Jun 1, 2014·The International Journal of Biochemistry & Cell Biology·Olena LykhmusMaryna Skok
Aug 15, 2014·BioMed Research International·Marion MeixnerVeronika Grau
Sep 29, 2017·Frontiers in Cellular Neuroscience·Olena LykhmusMaryna Skok
Jun 29, 2018·Frontiers in Pharmacology·Kateryna UspenskaMaryna Skok
Nov 5, 2020·The International Journal of Biochemistry & Cell Biology·Olena KalashnykMaryna Skok
May 18, 2021·Biochemical and Biophysical Research Communications·Olena KalashnykMaryna Skok

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