Abstract
The functional consequence of the casein kinase I-catalyzed phosphorylation of the lens gap junctional protein connexin49 was investigated using a sheep primary lens cell culture system. To determine whether the phosphorylation of connexin49 catalyzed by endogenous casein kinase I results in an altered junctional communication between lens cells, the effect of the casein kinase I-specific inhibitor CKI-7 on Lucifer Yellow dye transfer between cells in the lens culture was examined. Dye transfer was analyzed in cultures of different ages because we have demonstrated previously that the expression of connexin49 increases as the cultures age while that of connexin43, which is likely not a substrate for casein kinase I, has been shown to decrease [Yang & Louis (1999) Invest. Ophthalmol. Vis. Sci. 41: 2568-2564]. In 9-day old lens cultures, in which gap junctions are composed primarily of connexin43, CKI-7 had little effect on the rate of dye transfer between lens cells. In contrast, treatment of 15-day and 28-day old cultures with CKI-7 resulted in a significant increase in the rate of dye transfer. Thus, the extent of this CKI-7-dependent increase in cell-to-cell communication was positively correlated with the level of expression...Continue Reading
Citations
Apr 28, 2004·The International Journal of Biochemistry & Cell Biology·Paul D Lampe, Alan F Lau
Feb 19, 2002·Kidney International·Helga VitzthumKonrad Wolf
Feb 19, 2002·Kidney International·Marina V MikhailovaThomas E Andreoli
Feb 22, 2001·Annual Review of Physiology·W B ReevesT E Andreoli
Feb 25, 2018·Acta Physiologica·H Dimke, J Schnermann
Sep 25, 2003·Physiological Reviews·Juan C SaezEric C Beyer
Aug 28, 2003·Acta Physiologica Scandinavica·J C SáezM V L Bennett
Jul 24, 2008·Cell Communication & Adhesion·Xinye YinJean X Jiang
Oct 4, 2008·Antioxidants & Redox Signaling·Viviana M Berthoud, Eric C Beyer
Jan 17, 2009·Journal of Genetics·Patricia V AgostinoDiego A Golombek
Aug 23, 2020·International Journal of Molecular Sciences·Viviana M BerthoudEric C Beyer
May 18, 2007·Progress in Biophysics and Molecular Biology·Alonso P Moreno, Alan F Lau
Apr 2, 2011·The Journal of Biological Chemistry·Jialu LiuJean X Jiang
Sep 25, 2002·The Journal of Biological Chemistry·Cynthia D Cooper, Paul D Lampe
Jun 16, 2005·Biochimica Et Biophysica Acta·Alonso P Moreno