Functional evidence for a glibenclamide-sensitive K+ channel in rat ileal smooth muscle

European Journal of Pharmacology
H FranckH D Allescher

Abstract

The motor activity of gastrointestinal smooth muscle is closely related to the membrane potential. Controlling the membrane potential via modulation of K+ channels is essential for the action of neurotransmitters on smooth muscle. In the present study the effect of the K+ channel activator, lemakalim, on longitudinal smooth muscle of the rat ileum was investigated. Segments of rat ileum were stimulated by the muscarinic receptor agonist, carbachol (10(-6) M). Lemakalim (10(-10) to 3 x 10(-5) M) induced a dose-dependent inhibition of the carbachol-induced contraction. This inhibitory effect of lemakalim was not modified by neural blockade with tetrodotoxin (10(-6) M, n = 9). Glibenclamide (10(-7) to 10(-5) M), a specific blocker of ATP-dependent K+ channels antagonized dose dependently the relaxant effect of lemakalim (IC50: 3.4 x 10(-6) M, n = 11, P < 0.001). In contrast, apamin (10(-7) M, n = 9, n.s.) and charybdotoxin (10(-7) M, n = 9, n.s.), specific blockers of Ca2+-dependent K+ channels and the non-specific K+ channel blocker, tetraethylammonium (10(-4) to 10(-1) M), had no influence on the inhibitory effect of lemakalim. Contractions induced by the Ca2+ channel activator, Bay-K-8644, were completely inhibited by lemakalim...Continue Reading

References

Dec 1, 1992·British Journal of Pharmacology·H SakutaY Watanabe
Aug 1, 1992·Pflügers Archiv : European journal of physiology·Z FanM Hiraoka
Jan 1, 1992·Pflügers Archiv : European journal of physiology·S D Silberberg, C van Breemen
May 1, 1992·The American Journal of Physiology·F F SamahaR S Balaban
May 1, 1992·Pflügers Archiv : European journal of physiology·A CarlJ R Hume
Feb 1, 1991·The American Journal of Physiology·J M PostJ R Hume
Aug 1, 1988·Pflügers Archiv : European journal of physiology·M L AshfordC N Hales
Sep 1, 1989·Pflügers Archiv : European journal of physiology·D EscandeI Cavero
Dec 19, 1989·European Journal of Pharmacology·A den HertogA Nelemans
Jan 1, 1989·Pflügers Archiv : European journal of physiology·D Escande
Nov 1, 1989·Trends in Pharmacological Sciences·U Quast, N S Cook
Jan 1, 1988·British Journal of Pharmacology·N S Cook, R P Hof
Feb 15, 1993·European Journal of Pharmacology·H Sakuta, K Okamoto

❮ Previous
Next ❯

Citations

Nov 3, 2006·European Journal of Nutrition·Arif-ullah Khan, Anwarul Hassan Gilani
Dec 12, 1995·European Journal of Pharmacology·R PoggioliA Bertolini
Oct 21, 2011·Natural Product Research·Arif-ullah Khan, Anwarul Hassan Gilani
Apr 1, 1999·Annual Review of Physiology·B HorowitzK M Sanders
May 17, 2011·The Journal of Surgical Research·Michael S KasparekMichael G Sarr
Apr 19, 2015·Journal of Ethnopharmacology·Amber Hanif Palla, Anwarul-Hassan Gilani
Jul 1, 2008·Journal of Ethnopharmacology·Anwarul Hassan GilaniSheikh Arshad Saeed
Nov 2, 2006·Basic & Clinical Pharmacology & Toxicology·Anwarul Hassan GilaniJoachim W Herzig
Apr 18, 2015·BMC Complementary and Alternative Medicine·Malik Hassan MehmoodAnwarul Hassan Gilani
Mar 22, 2001·Journal of Autonomic Pharmacology·F Vogalis
Jul 13, 2018·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ban WangYasuyuki Tanahashi
Dec 26, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jae Hoon SimKi Whan Kim
Aug 9, 2020·Journal of Experimental Pharmacology·Amber Hanif PallaMebrahtom Gebrelibanos Hiben

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.