Functional evidence for a telomerase repressor gene on human chromosome 10p15.1

Oncogene
A NishimotoMitsuo Oshimura

Abstract

Based on the sites of frequent allelic loss in hepatocellular carcinoma, five normal human chromosomes (2, 4, 5, 10 and 16) were transferred individually into a telomerase-positive human hepatocellular carcinoma cell line, Li7HM, by microcell-mediated chromosome transfer (MMCT). Chromosome 10, but not the others, repressed telomerase activity immediately and stopped cell growth after 50 population doublings (PDs). Loss of the transferred 10p loci resulted in the emergence of revertant cells that continued to proliferate and expressed telomerase activity, suggesting the presence of a telomerase repressor gene on this chromosomal arm. Transfer of a series of defined fragments from chromosome 10p successfully narrowed down the responsible region: a 28.9-cM region on 10p15 (between WI-4752 and D10S249), but not a 26.2-cM region (between D10S1728 and D10S249), caused repression of telomerase activity and progressive telomere shortening. A strong correlation between the expression level of telomerase catalytic subunit gene (hTERT) and telomerase activity was observed. These findings suggest that a novel telomerase repressor gene which controls the expression of hTERT is located on the 2.7-cM region (between WI-4752 and D10S1728) on c...Continue Reading

References

May 31, 1990·Nature·C B HarleyC W Greider
Oct 15, 1985·Cancer·S C BernsteinH J Weinstein
Oct 1, 1995·Japanese Journal of Cancer Research : Gann·H OhmuraM Oshimura
Sep 1, 1995·Science·J FengJ Yu
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·T de Lange
Jun 1, 1996·Genomics·A C KimmelmanB C Liang
Feb 14, 1997·Science·L HarringtonM O Robinson
Jan 1, 1997·Cancer Genetics and Cytogenetics·N MiuraM Oshimura
Apr 1, 1997·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M Oshimura, J C Barrett
Sep 17, 1998·Genes, Chromosomes & Cancer·H TanakaM Oshimura
Jan 16, 1999·Journal of the National Cancer Institute·A P CuthbertR F Newbold
Sep 2, 1999·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·H KugohM Oshimura
Dec 10, 1999·British Journal of Cancer·T J HulsebosC C Tijssen

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Citations

Dec 4, 2002·Genes, Chromosomes & Cancer·Hiroyuki KugohMitsuo Oshimura
Dec 18, 2001·The Journal of Pathology·W N KeithR M Glasspool
Jun 12, 2002·The American Journal of Pathology·Renske D M SteenbergenPeter J F Snijders
Aug 25, 2006·Nature Reviews. Cancer·Paraskevi A Farazi, Ronald A DePinho
May 25, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J FajkusJ Maláska
Feb 9, 2011·Molecular and Cellular Biology·Dong-Lai QiHiroyuki Kugoh
Sep 2, 2005·Molecular and Cellular Biology·Masahiro TakakuraJerry W Shay
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Yu-Sheng CongJerry W Shay
Aug 30, 2005·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·H ShimaK Chayama
Feb 4, 2009·BMC Molecular Biology·Norimasa MiuraJunichi Hasegawa
Jan 6, 2016·Cancers·Hiroyuki KugohMitsuo Oshimura
Feb 3, 2005·International Journal of Cancer. Journal International Du Cancer·Hiromi TanakaMitsuo Oshimura
Jun 7, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Motoko SasakiYasuni Nakanuma
Nov 24, 2004·Biochemical and Biophysical Research Communications·Tomoki NakajimaTakeshi Okanoue
Jul 14, 2010·Biochemical and Biophysical Research Communications·Dong-Lai QiHiroyuki Kugoh
Feb 7, 2015·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Mitsuo OshimuraToshiaki Inoue
Feb 19, 2002·Oncogene·Anne-Lyse DucrestMarkus Nabholz
Apr 26, 2006·The American Journal of Gastroenterology·Prodromos Hytiroglou, Neil D Theise
May 15, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Joern KamradtBernd Wullich
Feb 25, 2020·Experimental Cell Research·Teruhiko SuzukiMitsuo Oshimura

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