PMID: 7086680Jun 1, 1982Paper

Functional evidence for the presence of cholinergic nerve endings in the colonic mucosa of the rat

The Journal of Pharmacology and Experimental Therapeutics
Z C WuT S Gaginella

Abstract

Uptake of [3H]choline and the subsequent synthesis and release of [3H]acetylcholine was studied in a preparation of rat colonic mucosa in vitro. [3H]Choline uptake was inhibited by hemicholinium-3. Release of synthesized [3H]acetylcholine was increased significantly over base line by 50 mM K+ and 100 microM veratridine. K+-evoked release was inhibited by 83% in tissue preincubated (during [3H]choline uptake) in Na+-free media. The effect of K+ was also blocked in Ca++-free media by 15 mM Mg++ and 0.1 mM verapamil. Release from the longitudinal and circular muscle of the colon evoked by K+ and veratridine was greater than release from the mucosa. Tetrodotoxin (0.2 microM) did not affect K+-induced release of [3H] acetylcholine but significantly blocked veratridine-induced release from the mucosal (by 63%) and muscle (by 68%) preparations. The data confirm that functional cholinergic nerve endings exist in close proximity to colonic epithelial cells.

Related Concepts

Acetylcholine Sulfate (1: 1)
Calcium
Choline Hydroxide
Taenia Coli
Hemicholinium 3
Structure of Intestinal Gland
Magnesium
Nerve Endings
Parasympathetic Nervous System
August Rats

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Generating Insulin-Secreting Cells

Reprogramming cells or using induced pluripotent stem cells to generate insulin-secreting cells has significant therapeutic implications for diabetics. Here is the latest research on generation of insulin-secreting cells.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Epigenome Editing

Epigenome editing is the directed modification of epigenetic marks on chromatin at specified loci. This tool has many applications in research as well as in the clinic. Find the latest research on epigenome editing here.