PMID: 11606206Oct 19, 2001Paper

Functional expression and characterization of the cytoplasmic aminopeptidase P of Caenorhabditis elegans

European Journal of Biochemistry
V LaurentR Elwyn Isaac

Abstract

Aminopeptidase P (AP-P; X-Pro aminopeptidase; EC 3.4.11.9) cleaves the N-terminal X-Pro bond of peptides and occurs in mammals as both cytosolic and plasma membrane forms, encoded by separate genes. In mammals, the plasma membrane AP-P can function as a kininase, but little is known about the physiological role of the cytosolic enzyme. The C. elegans genome contains a single gene encoding AP-P (W03G9.4), analysis of which predicts regions displaying high levels of amino-acid sequence homology between the predicted gene product and mammalian cytoplasmic AP-P, with the absolute conservation of key catalytic residues. The sequence of an EST (yk91g4), comprising the open reading frame of W03G9.4, confirmed the predicted genomic structure of the gene and the prediction that W03G9.4 codes for a nonsecreted protein with a molecular mass of 68 kDa. Nematodes transformed with a promoter reporter construct, W03G9.4:GFP, showed high levels of fluorescence in the intestine of larvae and adult hermaphrodites, indicating that the intestine is a major site of W03G9.4 expression. yk91g4 tagged with a hexahistidine and DLYDDDDK peptide epitope was expressed in Escherichia coli to yield, after affinity purification, a recombinant protein with a ...Continue Reading

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Citations

Jul 4, 2009·The Journal of Biological Chemistry·Daniel RaghebMichael Klemba
Nov 8, 2012·Biochemical and Biophysical Research Communications·Sang Ho YoonMyoung-Hwan Kim
Dec 6, 2005·Neuroscience Letters·Shobha BhargavaDileep Nandkumar Deobagkar
Oct 12, 2005·International Journal for Parasitology·Catherine J LilleyPeter E Urwin
May 25, 2002·Developmental Biology·Morris F Maduro, Joel H Rothman
May 9, 2019·Cell and Tissue Research·Ivan Dimov, Morris F Maduro
Nov 7, 2007·Archives of Biochemistry and Biophysics·Stephen C Graham, J Mitchell Guss
Dec 12, 2002·Chemical Reviews·W Todd Lowther, Brian W Matthews

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