Functional expression and germline transmission of a human chromosome fragment in chimaeric mice

Nature Genetics
Kazuma TomizukaIsao Ishida

Abstract

Human chromosomes or chromosome fragments derived from normal fibroblasts were introduced into mouse embryonic stem (ES) cells via microcell-mediated chromosome transfer (MMCT) and viable chimaeric mice were produced from them. Transferred chromosomes were stably retained, and human genes, including immunoglobulin (Ig) kappa, heavy, lambda genes, were expressed in proper tissue-specific manner in adult chimaeric tissues. In the case of a human chromosome (hChr.) 2-derived fragment, it was found to be transmitted to the offspring through the germline. Our study demonstrates that MMCT allows for introduction of very large amounts of foreign genetic material into mice. This novel procedure will facilitate the functional analyses of human genomes in vivo.

References

Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·K IllmenseeC M Croce
Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R E Fournier, F H Ruddle
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·M J DeweyB Mintz
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·K Illmensee, C M Croce
Jun 21, 1990·Biochimica Et Biophysica Acta·R V Trask, J J Billadello
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K Yamauchi-TakiharaC C Liew
Dec 1, 1987·Cell Structure and Function·K KatohH Kondoh
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M W Berchtold, A R Means
Nov 1, 1983·The Journal of Experimental Zoology·A GroppC P Claussen
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C J EpsteinD R Cox
Dec 14, 1995·Nature·R Ramírez-SolisA Bradley
Aug 4, 1995·Biochemical and Biophysical Research Communications·S WatanabeT Yagi
Jul 1, 1993·European Journal of Immunology·S C Williams, G Winter
Oct 1, 1993·Analytical Biochemistry·T YagiS Aizawa
Dec 1, 1993·European Journal of Immunology·R KleinH G Zachau
Dec 1, 1993·European Journal of Immunology·R Klein, H G Zachau
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·R HellerW R Brown
Jun 1, 1996·Current Opinion in Genetics & Development·W BrownA Chand
Jun 1, 1996·Current Opinion in Genetics & Development·W BrownA Chand

❮ Previous
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Citations

Sep 25, 1999·Molecular Reproduction and Development·S MuellerG Brem
Mar 10, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D Schindelhauer
Aug 10, 2006·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Jason D Heaney, Sarah K Bronson
Sep 1, 2005·Chromosoma·Brenda R Grimes, Zoia Larin Monaco
Oct 1, 2013·Cell and Tissue Research·Jan M Deussing
Mar 16, 2013·Cytotechnology·Narumi UnoMitsuo Oshimura
Dec 5, 2006·Stem Cell Reviews·Xianying RenMitsuo Oshimura
Dec 5, 2002·Archives of Biochemistry and Biophysics·Frank J Gonzalez, Shioko Kimura
Sep 22, 2001·Physiology & Behavior·M DierssenX Estivill
Apr 12, 2000·Trends in Biotechnology·W R BrownM Hong Shen
Oct 4, 2000·Nature Biotechnology
Nov 26, 1998·Journal of Biotechnology·E WolfG Brem
Jun 5, 2002·Trends in Genetics : TIG·Zoia Larin, José E Mejía
Sep 1, 1997·Trends in Genetics : TIG·C Huxley
Jan 10, 1998·Trends in Genetics : TIG·E M Rubin, D J Smith
Jun 4, 1999·Trends in Genetics : TIG·D Hernandez, E M Fisher
Feb 6, 2003·Gene·H J LippsJ Bode
Dec 17, 2002·Current Opinion in Biotechnology·Sirid Aimée Kellermann, Larry L Green
Dec 17, 2002·Current Opinion in Biotechnology·Louis Marie Houdebine
Aug 5, 1998·Current Opinion in Genetics & Development·J M Vos
May 30, 2001·Trends in Molecular Medicine·K H Choo
Jul 10, 2003·Lancet Neurology·Majid HafezparastElizabeth M C Fisher
Jul 17, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Michele M P LufinoRichard Wade-Martins
Nov 27, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Hidetoshi HoshiyaMitsuo Oshimura
Dec 10, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yasuhiro KazukiMitsuo Oshimura
Jul 14, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yasuhiro Kazuki, Mitsuo Oshimura
Sep 3, 2002·Nature Biotechnology·Yann Echelard, Harry Meade
Sep 10, 2005·Nature Biotechnology·Nils Lonberg
Nov 14, 1997·Nature Biotechnology·J M Vos
Aug 13, 2002·Nature Biotechnology·Yoshimi KuroiwaJames M Robl
Jun 1, 1997·Nature Genetics·S Rastan
Nov 7, 2006·Nature Methods·Hiroyuki MatsumuraTakashi Tada
May 15, 2002·Cloning and Stem Cells·Isao IshidaNils Lonberg
Aug 28, 2010·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Yuichi IidaMitsuo Oshimura
Jul 13, 1999·Human Molecular Genetics·C GuiducciP Donini

❮ Previous
Next ❯

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