PMID: 6987664Feb 1, 1980Paper

Functional expression in yeast of the Escherichia coli plasmid gene coding for chloramphenicol acetyltransferase

Proceedings of the National Academy of Sciences of the United States of America
J D CohenJ Marmur

Abstract

The Escherichia coli R factor-derived chloramphenicol resistance (camr) gene is functionally expressed in the yeast Saccharomyces cerevisiae. the gene was introduced by transformation into yeast cells as part of a chimeric plasmid, pYT11-LEU2, constructed in vitro. The plasmide vector consists of the E. coli plasmid pBR325 (carrying the camr gene), the yeast 2-micron DNA plasmid, and the yeast LEU2 structural gene. Yeast cells harboring pYT11-LEU2 acquire resistance to chloramphenicol and cell-free extracts prepared from such cells contain chloramphenicol acetyltransferase (acetyl-CoA: chloramphenicol 3-O-acetyltransferase, EC 2.3.1.28), the enzyme specified by the camr gene in E. coli. Resistance to chloramphenicol and the presence of chloramphenicol acetyltransferase activity segregate with the yeast marker LEU2, carried by the transforming plasmid, during both mitotic growth and meiotic division.

References

Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D VapnekS R Kushner
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B Ratzkin, J Carbon
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A HinnenG R Fink
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·K StruhlR W Davis
Feb 1, 1978·Antimicrobial Agents and Chemotherapy·W V ShawF W Goldstein
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K StruhlR W Davis
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·C P HollenbergH D Royer

❮ Previous
Next ❯

Citations

Jan 1, 1981·Molecular & General Genetics : MGG·J B McNeil, J D Friesen
May 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·T HiroseB Sato
Oct 24, 2013·Biochemical Engineering Journal·Mary Alice SalazarSarah W Harcum
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R RoggenkampC P Hollenberg
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·K HughesN H Giles
Apr 15, 1983·Biochemical and Biophysical Research Communications·D J BallanceG Turner
Jul 16, 1982·Biochemical and Biophysical Research Communications·P F Lurquin, A Kleinhofs
Feb 1, 1990·Differentiation; Research in Biological Diversity·T HasegawaS Sekiya
Dec 31, 1987·Biochemical and Biophysical Research Communications·J W KwakM H Han
Aug 14, 1981·Biochemical and Biophysical Research Communications·D UrsicJ P Helgeson
Jan 1, 1983·CRC Critical Reviews in Biochemistry·W V Shaw
Jan 1, 1985·Biotechnology & Genetic Engineering Reviews·S M KingsmanN A Roberts
Jan 1, 1983·Nutrition Reviews·C D Earl, F M Ausubel
Mar 3, 1983·Nature·R M WalmsleyT D Petes
Nov 30, 2012·Biopolymers·Antônio Américo Barbosa VianaMaria Fátima Grossi-de-Sá
Sep 1, 1991·Molecular & General Genetics : MGG·B K Oh, D Apirion
Jan 1, 1994·Antisense Research and Development·D Atkins, W L Gerlach
Feb 1, 1992·Journal of Bacteriology·C RensingB Friedrich
Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.