PMID: 11903623Mar 21, 2002Paper

Functional expression of a locust tyramine receptor in murine erythroleukaemia cells

Insect Molecular Biology
J PoelsJ Vanden Broeck

Abstract

The LCR/MEL system (Locus Control Region/Murine Erythroleukaemia cells) was employed to express and characterize the Locusta migratoria tyramine receptor (TyrLoc), an insect G protein-coupled receptor. Functional agonist-dependent responses were recorded in stable, tyramine receptor expressing cell clones (MEL-TyrLoc). Tyramine elicited a dose-dependent increase of cytosolic Ca2+-ions and an attenuation of forskolin-induced cyclic adenosine monophosphate (AMP) production. Octopamine was shown to be a weak agonist for both responses. In addition, yohimbine proved to be a potent tyramine receptor antagonist. This study reports the first application of the LCR/MEL expression system in functional assays for G protein-coupled receptors and therefore expands the capabilities of this system by exploiting the functionality of the signal transduction pathways.

References

Jan 1, 1996·International Review of Cytology·J J Vanden Broeck
Jan 1, 1995·Journal of Receptor and Signal Transduction Research·M AmarI Bermudez
Sep 1, 1996·Insect Biochemistry and Molecular Biology·E von Nickisch-RosenegkH Breer
Feb 1, 1997·Molecular Pharmacology·C C GerhardtH Van Heerikhuizen
Jan 7, 1999·European Journal of Pharmacology·C I HolmbergK E Akerman
Feb 27, 2001·Insect Biochemistry and Molecular Biology·W Van PoyerJ Vanden Broeck

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Citations

Apr 28, 2004·Insect Biochemistry and Molecular Biology·Jeroen PoelsJozef Vanden Broeck
Apr 15, 2004·Journal of Insect Physiology·Andrew Donini, Angela B Lange
Jul 14, 2010·Journal of Insect Physiology·Heleen VerlindenJozef Vanden Broeck
Jul 1, 2008·General and Comparative Endocrinology·Angela B Lange
Sep 25, 2007·Pharmacology & Therapeutics·David K Grandy
Jan 27, 2005·The Journal of Comparative Neurology·Julie A MustardAlison R Mercer
Jun 30, 2005·Archives of Insect Biochemistry and Physiology·Goudarz MolaeiAngela B Lange
Mar 15, 2005·Insect Biochemistry and Molecular Biology·Essam E Enan
Nov 9, 2005·Biochemical and Biophysical Research Communications·Giuseppe CazzamaliCornelis J P Grimmelikhuijzen
Dec 3, 2014·Insect Biochemistry and Molecular Biology·Heleen VerlindenAlison Mercer
Nov 22, 2002·American Journal of Physiology. Cell Physiology·Edward M Blumenthal
Jul 28, 2018·Frontiers in Physiology·Vaishnavi SukumarPäivi H Torkkeli
Jun 13, 2020·Frontiers in Physiology·Fan LiTong-Xian Liu
May 8, 2007·Neurochemical Research·Tahira Farooqui
Mar 11, 2021·The Journal of Experimental Biology·Luca FinettiGiovanni Bernacchia
Apr 29, 2021·Journal of Neurogenetics·Melissa A WhiteMariana F Wolfner
Apr 30, 2021·The Journal of Experimental Biology·Luca FinettiGiovanni Bernacchia
May 1, 2021·Insects·Luca FinettiGiovanni Bernacchia

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