PMID: 8990205Jan 7, 1997Paper

Functional expression of a novel human neurokinin-3 receptor homolog that binds [3H]senktide and [125I-MePhe7]neurokinin B, and is responsive to tachykinin peptide agonists

Proceedings of the National Academy of Sciences of the United States of America
J E KrauseV V Karpitskiy

Abstract

In 1992, Xie et al. identified a cDNA sequence in the expression cloning search for the kappa opioid receptor. When the cDNA was expressed in Cos-7 cells, binding of opioid compounds was observed to be of low affinity and without kappa, mu, or delta selectivity [Xie, G.-X., Miyajima, A. and Goldstein, A. (1992) Proc. Natl. Acad. Sci. USA 89, 4124-4128]. This cDNA was highly homologous to the human neurokinin-3 (NK-3) receptor sequence, and displayed lower homology to NK-1 and NK-2 sequences. This sequence was stably expressed in Chinese hamster ovary cells, which do not express neurokinin receptors naturally, and ligand binding and second messenger characteristics were compared with a human NK-3 receptor. The NK-3 receptor homolog bound [3H] senktide with a Kd of 39 nM, similar to that of the NK-3 receptor. The rank order of tachykinin peptides competing for [3H]senktide binding at the NK-3 receptor homolog was [MePhe7]neurokinin B > senktide > substance P = neurokinin A > neurokinin B. This cell line also bound [125I-MePhe7]neurokinin B; however, neurokinin B was an effective competitor. Tachykinin peptides stimulated both inositol phospholipid hydrolysis and arachidonic acid release at NK-3 and NK-3 receptor homolog cell line...Continue Reading

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Citations

Jan 20, 2004·Life Sciences·Jocelyn N PennefatherCarlo Alberto Maggi
Nov 8, 2001·European Journal of Pharmacology·R Patacchini, C A Maggi
Feb 28, 2002·European Journal of Pharmacology·Nigel M Page, Nicola J Bell
Aug 12, 1999·European Journal of Pharmacology·A Saria
Apr 29, 2000·Trends in Pharmacological Sciences·C A Maggi
Mar 7, 2001·Regulatory Peptides·N M PageP J Lowry
May 30, 2001·The International Journal of Biochemistry & Cell Biology·S Harrison, P Geppetti
Mar 7, 1998·British Journal of Pharmacology·J MagranerM L Candenas
Apr 17, 2004·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·S C BischoffM Schemann
Jul 23, 2003·British Journal of Pharmacology·Chiara DianzaniRoberto Fantozzi
Sep 10, 2014·The Journal of Allergy and Clinical Immunology·Tina L SumpterAdriana T Larregina
Feb 13, 2014·British Journal of Pharmacology·Stephen P H AlexanderUNKNOWN CGTP Collaborators
Jul 30, 2019·Frontiers in Endocrinology·Naama MizrahiBerta Levavi-Sivan
Nov 14, 1997·Trends in Pharmacological Sciences·C A Maggi, T W Schwartz

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