Abstract
A cDNA encoding a 100-kDa subunit (XenNR1) of the N-methyl-D-aspartate (NMDA) glutamate receptor type has been cloned from Xenopus central nervous system. When XenNR1 is coexpressed in a mammalian cell line with a recently cloned 51-kDa non-NMDA receptor subunit (XenU1), also from Xenopus, it forms a functional unitary receptor exhibiting the pharmacological properties characteristic of both NMDA and non-NMDA receptors. Firstly, XenU1 can replace NR2 subunits, in complementing XenNR1 to introduce the ligand binding properties of a complete NMDA receptor. Second, responses to both NMDA and non-NMDA receptor agonists and antagonists were obtained in patch-clamp recordings from the cotransfected cells, but no significant responses were recorded when the cells were singly transfected. Third, from solubilized cell membranes from the cotransfected cells, an antibody to the NR1 subunit coprecipitated the binding sites of the non-NMDA receptor subunit. The unitary glutamate receptor has a unique set of properties that denote intersubunit interaction, including a glycine requirement for the responses to non-NMDA as well as to NMDA receptor agonists and voltage-dependent block by Mg2+ of the non-NMDA agonist responses.
References
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
May 22, 1992·Science·H MonyerP H Seeburg
Jun 30, 1992·Biochemical and Biophysical Research Communications·H SugiharaS Nakanishi
May 7, 1992·Nature·H MeguroM Mishina
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J M HenleyE A Barnard
Jul 2, 1992·Nature·T KutsuwadaM Arakawa
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·G M DurandR S Zukin
Apr 1, 1992·Pflügers Archiv : European journal of physiology·P F MéryR Fischmeister
Nov 7, 1991·Nature·K MoriyoshiS Nakanishi
May 1, 1987·The Journal of Physiology·V VlachováF Vyskocil
Dec 7, 1989·Nature·P GregorV I Teichberg
Jan 1, 1989·Journal of Neurochemistry·J M Henley, E A Barnard
Dec 7, 1989·Nature·K WadaY Nakatani
Aug 5, 1988·Science·T HonoréF E Nielsen
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E H WongL L Iversen
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Aug 1, 1993·Trends in Pharmacological Sciences·P H Seeburg
Dec 1, 1994·Neuron·Y Stern-BachS F Heinemann
Aug 16, 1994·European Journal of Pharmacology·D J Laurie, P H Seeburg
Sep 22, 1995·Science·H C KornauP H Seeburg
May 1, 1993·Neuron·M HollmannS Heinemann
Feb 1, 1995·Journal of Neurochemistry·S GrimwoodP J Whiting
Dec 15, 1993·The Biochemical Journal·M CikF A Stephenson
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·Z G Wo, R E Oswald
Jan 1, 1993·Neuropharmacology·P MarinJ Bockaert
Mar 10, 1994·Nature·M ShengL Y Jan
Jan 1, 1994·Annual Review of Neuroscience·M Hollmann, S Heinemann
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G M DurandR S Zukin
Sep 15, 1993·Gene·R L FoldesR K Kamboj
Citations
Jan 15, 2009·Invertebrate Neuroscience : in·Kathryn B GreyBrian D Burrell
Sep 5, 1998·Brain Research·N YaghoubiD H Farb
Mar 24, 2000·European Journal of Pharmacology·M MugnainiB Bunnemann
May 1, 1997·Trends in Pharmacological Sciences·E A Barnard
Apr 2, 1998·Progress in Neurobiology·E K Michaelis
Aug 28, 1999·Progress in Neurobiology·T Yamakura, K Shimoji
Jan 18, 2002·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Katherine M FlynnMartin P Schreibman
Jan 8, 2008·Journal of Molecular Biology·Carsten Schmidt, Michael Hollmann
Jan 30, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Der-Wang TzengWei-Yuan Chow
Apr 25, 2000·Neuron·A FayyazuddinJ Neyton
May 19, 2009·Journal of Molecular Biology·Carsten SchmidtMichael Hollmann
Feb 8, 2003·Journal of Neurophysiology·Erik Harvey-Girard, Robert J Dunn
May 16, 2007·The Journal of Pharmacology and Experimental Therapeutics·C Thetford Smothers, John J Woodward
Oct 11, 2005·Molecular Pharmacology·Carsten SchmidtMichael Hollmann
Jan 26, 2002·Molecular Pharmacology·Tim GreenStephen F Heinemann
Sep 23, 2003·Clinical Chemistry·Svetlana A DambinovaAlexander A Skoromets
Jul 5, 2003·Physiological Reviews·Ralf M LoselMartin Wehling
Nov 21, 1997·Journal of Molecular Biology·M M Soloviev, E A Barnard