PMID: 11902119Mar 21, 2002Paper

Functional expression of corticotropin-releasing hormone (CRH) receptor 1 in cultured rat microglia

Journal of Neurochemistry
Wei WangKimberly E Dow

Abstract

Corticotropin-releasing hormone (CRH), known as a key regulator of the hypothalamic-pituitary-adrenal axis response to stress, elicits its biological effects by binding to two membrane receptors (CRH-R1 and CRH-R2). The present studies examined the presence of functional expression of CRH receptors in cultured microglia of rat. CRH-R1 mRNA and protein were detected by reverse transcriptase polymerase chain reaction (RT-PCR), western blotting and receptor chemical cross-linking assay in cultured microglia. CRH-R2 mRNA was undetectable by RT-PCR. The radioligand binding analysis using [125I]Tyr-rat/human CRH revealed a high affinity binding site (Kd of 1.2 nm and Bmax of 84 fmol/mg of protein). Competition studies using CRH and related peptides indicated kinetic and pharmacological characteristics consistent with the CRH-R1 receptor subtype. Receptor chemical cross-linking assay demonstrated a single band of CRH receptor with a molecular weight of -77 kDa, which was inhibited in the presence of excess unlabeled rat/human CRH in a dose-dependent manner and inhibited by a CRH receptor antagonist astressin. Functional coupled cAMP production in cultured microglia was stimulated by exogenous addition of CRH and related peptides in a ...Continue Reading

Citations

Sep 17, 2009·Acta Neuropathologica·Beatriz Gómez-González, Alfonso Escobar
Aug 9, 2006·Journal of Neurochemistry·Jiyeon OckKyoungho Suk
Dec 22, 2009·Journal of Neuroscience Research·Hongbin YuanXueyin Shi
May 18, 2010·Neuropharmacology·Michele HummelGarth T Whiteside
Aug 19, 2016·CNS Neuroscience & Therapeutics·Xiao-Lin YangShi-Yong Liu
Apr 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christina BenouSamia J Khoury
Mar 1, 2019·Nature Metabolism·Martin ValdearcosSuneil K Koliwad
Mar 1, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Muriel LaraucheJames A McRoberts
Aug 21, 2019·Frontiers in Cellular Neuroscience·Theoharis C TheoharidesMona Bawazeer
Feb 15, 2020·International Journal of Molecular Sciences·Giuseppe BanfiEva Reali
Oct 18, 2020·Science China. Life Sciences·Xiaoning JiaHailan Hu
Feb 16, 2021·Neurochemistry International·Katherine PicardMarie-Ève Tremblay
Apr 14, 2021·BioFactors·Theoharis C TheoharidesAntonios Politis

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