Functional Expression of Electron Transport Chain and FoF1-ATP Synthase in Optic Nerve Myelin Sheath

Neurochemical Research
Martina BartolucciIsabella Panfoli

Abstract

Our previous studies reported evidence for aerobic ATP synthesis by myelin from both bovine brainstem and rat sciatic nerve. Considering that the optic nerve displays a high oxygen demand, here we evaluated the expression and activity of the five Respiratory Complexes in myelin purified from either bovine or murine optic nerves. Western blot analyses on isolated myelin confirmed the expression of ND4L (subunit of Complex I), COX IV (subunit of Complex IV) and β subunit of F1Fo-ATP synthase. Moreover, spectrophotometric and in-gel activity assays on isolated myelin, as well as histochemical activity assays on both bovine and murine transversal optic nerve sections showed that the respiratory Complexes are functional in myelin and are organized in a supercomplex. Expression of oxidative phosphorylation proteins was also evaluated on bovine optic nerve sections by confocal and transmission electron microscopy. Having excluded a mitochondrial contamination of isolated myelin and considering the results form in situ analyses, it is proposed that the oxidative phosphorylation machinery is truly resident in optic myelin sheath. Data may shed a new light on the unknown trophic role of myelin sheath. It may be energy supplier for the ax...Continue Reading

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Citations

Jun 3, 2015·Molecular Neurobiology·Silvia RaveraIsabella Panfoli
Mar 15, 2018·Biology of the Cell·Silvia RaveraGiuliana Leoncini
Apr 15, 2017·Clinical and Experimental Pharmacology & Physiology·Michael V Berridge, Jiri Neuzil
Apr 30, 2019·Journal of Biophotonics·Silvia RaveraAndrea Amaroli
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Oct 22, 2020·Open Biology·Alessandro Maria MorelliIsabella Panfoli
Sep 1, 2020·Reviews in the Neurosciences·Antonio GiudittaAdolfo Sadile
Jun 5, 2021·Journal of Neuroscience Research·Silvia RaveraIsabella Panfoli

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