Functional Expression of Gloeobacter Rhodopsin in Synechocystis sp. PCC6803

Photochemistry and Photobiology
Que ChenKlaas J Hellingwerf

Abstract

Proteorhodopsins are light-driven proton pumps that occur widespread in Nature, where they function predominantly in environments with high incident irradiance. Their maximal absorbance is usually in the blue range, but can be extended into the (far)red range of the electromagnetic spectrum. Because they can be expressed heterologously, they may be exploited in studies aimed at increasing the efficiency of photosynthesis. Here we report further studies toward this goal, by comparing the expression of two different bacterial rhodopsins (Proteorhodopsin and Gloeobacter rhodopsin) in the model cyanobacterium Synechocystis sp. PCC6803. In particular, we investigated the pigments bound by the respective apo-opsins, and the oligomeric state of the corresponding holo-rhodopsins, both in Escherichia coli and in the cyanobacterial membranes. We conclude that the two proton-pumping rhodopsins are predominantly present in an oligomeric state (hexamers for Proteorhodopsin and trimers for Gloeobacter rhodopsin). Furthermore, Gloeobacter rhodopsin is able to bind an antenna carotenoid (in addition to retinal) and has the highest pumping rate at given light intensity. However, its lower expression level will decrease its physiological effecti...Continue Reading

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Citations

Mar 13, 2019·Photochemistry and Photobiology·Srividya GanapathyWillem J de Grip
Feb 2, 2018·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Fusao Komada
Apr 16, 2019·Frontiers in Bioengineering and Biotechnology·Que ChenKlaas J Hellingwerf
Nov 2, 2019·Biochimica Et Biophysica Acta. Biomembranes·Srividya GanapathyWillem J de Grip

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