Functional expression of Na-Ca exchanger clones measured with the fluorescent Ca(2+)-indicating dye fluo-3

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
P P Schnetkamp

Abstract

The process of Ca2+ homeostasis is of prime importance to all cells because of the ubiquitous role of cytoplasmic Ca2+ as an intracellular messenger and the cytotoxicity of sustained elevated cytosolic Ca2+ concentrations. Two classes of plasma membrane proteins are responsible for maintaining cytosolic free Ca2+ in the submicromolar range against a very large electrochemical Ca2+ gradient across the plasma membrane, the ATP-driven Ca2+ pump and Na-Ca exchangers. Two types of Na-Ca exchangers are known, the 3Na:1Ca exchangers found in heart, brain, kidney, and most other tissues and the 4Na:1Ca+ 1K exchanger found in retinal rod and cone photoreceptors. Functional expression of Na-Ca(/K) exchangers is most often measured as 45Ca uptake in Na(+)-loaded cells or as Na-Ca exchange currents with the giant excised patch technique. In this study, two functional assays used to detect expression of the bovine heart Na-Ca exchanger in CHO cells are described. Both assays are based on measurements of cytosolic free Ca2+ with the fluorescent Ca(2+)-indicating dye fluo-3 and should be equally applicable in the study of functional expression of both Na-Ca and Na-Ca/K exchanger clones.

References

Jan 1, 1992·Annual Review of Physiology·U B Kaupp, K W Koch
Aug 1, 1992·Current Opinion in Cell Biology·K D Philipson, D A Nicoll
Jan 1, 1991·Annals of the New York Academy of Sciences·P P SchnetkampR T Szerencsei
Feb 1, 1990·The Journal of Membrane Biology·L Lagnado, P A McNaughton
Apr 1, 1986·The Journal of Physiology·P P Schnetkamp
Jan 1, 1989·Progress in Biophysics and Molecular Biology·P P Schnetkamp
Jul 1, 1989·The American Journal of Physiology·P P SchnetkampR T Szerencsei
Apr 1, 1993·The American Journal of Physiology·V PijuanJ B Smith
Oct 1, 1995·Cell Calcium·P P Schnetkamp

❮ Previous
Next ❯

Citations

Jul 15, 2016·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Donald B JyrwaVeena Tandon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.