Functional expression of pharaonis phoborhodopsin in Escherichia coli

FEBS Letters
K ShimonoN Kamo

Abstract

Pharaonis phoborhodopsin, the photoreceptor of the negative phototaxis of archaebacterial Natronobacterium pharaonis, was functionally expressed in the heterologous system of Escherichia coli. Flash-photolysis on a millisecond time scale indicated that the photochemical properties of ppR expressed in E. coli were the same as those of the native ppR in N. pharaonis. We concluded that the integral membrane protein ppR is correctly folded in vivo in the eubacterial E. coli membrane.

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Citations

Aug 3, 2005·Extremophiles : Life Under Extreme Conditions·Jie FengShuang-Jiang Liu
Sep 11, 2002·Journal of Molecular Biology·Thomas FriedrichErnst Bamberg
Jul 16, 2003·Journal of Molecular Biology·Silke Hippler-MreyenM Engelhard
Jul 2, 2003·Biophysical Chemistry·Maki SatoKatsutoshi Nitta
Aug 9, 2002·Journal of Photochemistry and Photobiology. B, Biology·Yuki SudoNaoki Kamo
Oct 15, 2013·Nature Methods·Jonathan T S HopperCarol V Robinson
Jun 1, 2010·Nature Structural & Molecular Biology·Antoine GautierDaniel Nietlispach
Aug 14, 2008·The Journal of Biological Chemistry·Meike DoebberHeinz-Jürgen Steinhoff
Oct 10, 2001·Journal of Bacteriology·K H JungJ L Spudich
Jul 31, 2010·Journal of Biomedicine & Biotechnology·Hideki KandoriYuji Furutani
Jan 8, 2014·Journal of Peptide Science : an Official Publication of the European Peptide Society·Marc DittmannMartin Engelhard
Oct 28, 2008·Trends in Biotechnology·Ethan T Johnson, Claudia Schmidt-Dannert
May 14, 2017·Photochemistry and Photobiology·Philipp OrekhovMartin Engelhard
Sep 30, 2004·Biophysical Journal·Keiichi InoueMasahide Terazima
Jan 18, 2006·Journal of Molecular Biology·Johann P KlareMartin Engelhard
Jun 23, 2015·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Satoko DoiYuki Sudo
Oct 31, 2002·Photochemistry and Photobiology·Masayuki IwamotoNaoki Kamo
Jul 19, 2001·Nature·O BéjàE F DeLong
Dec 1, 2006·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Hendrik NaumannDaniel H Murgida
Mar 20, 2010·Molecular BioSystems·Ryota AbeKouhei Tsumoto

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