Functional expression of soluble human interleukin-11 (IL-11) receptor alpha and stoichiometry of in vitro IL-11 receptor complexes with gp130.

The Journal of Biological Chemistry
P NeddermannG Paonessa

Abstract

The interleukin-6 (IL-6) family of cytokines activates signaling through the formation of either gp130 homodimers, as for IL-6, or gp130-leukemia inhibitory factor receptor (LIFR) heterodimers as for ciliary neurotrophic factor (CNTF), leukemia inhibitory factor, oncostatinM, and cardiotrophin-1. Recent in vitro studies with IL-6 and CNTF have demonstrated that higher order hexameric receptor complexes are assembled in which signaling chain dimerization is accompanied by the dimerization of both the cytokine molecule and its specific receptor alpha subunits (IL-6Ralpha or CNTFRalpha, respectively). IL-11 is a member of the IL-6 family and known to require gp130 but not LIFR for signaling. In this study we investigate the functional and biochemical composition of the IL-11 receptor complex. The human IL-11 receptor alpha-chain was cloned from a human bone marrow cDNA library. IL-11Ralpha was shown to confer IL-11 responsiveness to human hepatoma cells either by cDNA transfection or by adding a soluble form of the receptor (sIL11Ralpha) expressed in the baculovirus system to the culture medium. In vitro immunoprecipitation experiments showed that sIL11Ralpha specifically binds IL-11 and that binding is enhanced by gp130. Similarl...Continue Reading

References

Oct 23, 1992·Science·T KishimotoT Taga
Jul 5, 1991·Science·S DavisG D Yancopoulos
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M HattoriG H Fey
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S R PaulD A Williams
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Feb 1, 1987·Molecular and Cellular Biology·J R de WetS Subramani
Dec 1, 1985·Molecular and Cellular Biology·G I EvanJ M Bishop
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Jun 1, 1994·The Biochemical Journal·S Rose-John, P C Heinrich
Nov 1, 1993·Stem Cells·Y C Yang
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·M NarazakiT Taga

❮ Previous
Next ❯

Citations

May 1, 1997·Protein Science : a Publication of the Protein Society·R J SimpsonL D Ward
Jan 16, 1999·Brain Research. Molecular Brain Research·M ThierJ Weis
Jun 10, 1998·Cytokine & Growth Factor Reviews·T HiranoM Hibi
Jan 11, 2002·European Journal of Biochemistry·Xiao-Ming WangJean Content
Oct 3, 1999·European Journal of Biochemistry·I TackenG Müller-Newen
Nov 20, 1996·AIDS Research and Human Retroviruses·R GeleziunasW C Greene
Jan 26, 1999·Annals of the New York Academy of Sciences·W L Trepicchio, A J Dorner
Jul 17, 2003·Endocrinology·Nicholas Underhill-DayJohn K Heath
Dec 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Igal Louria-HayonTony Pawson
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·P MärzS Rose-John
Apr 29, 2015·Immunotherapy·Tracy L Putoczki, Matthias Ernst
Jan 13, 2015·Epigenetics : Official Journal of the DNA Methylation Society·Kevin V Morris
Apr 5, 2002·Chembiochem : a European Journal of Chemical Biology·Albert Jeltsch
Aug 19, 2000·The Journal of Biological Chemistry·V A BartonJ K Heath
Jul 9, 2010·Journal of Leukocyte Biology·Tracy Putoczki, Matthias Ernst
Aug 30, 2008·Critical Reviews in Biochemistry and Molecular Biology·Joke Baute, Anne Depicker
Apr 3, 2019·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Atsushi YonejimaShuichi Kaneko
Jan 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S A JonesG M Fuller
Jun 17, 2000·Endocrine Reviews·C J Auernhammer, S Melmed
Sep 3, 2016·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Akira SassaManabu Yasui
Jan 23, 1999·International Reviews of Immunology·M Hibi, T Hirano
Nov 13, 2019·Seminars in Cancer Biology·Pathum S ThilakasiriAshwini L Chand

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.