PMID: 11607110Oct 1, 1990Paper

Functional expression of the Chlorella hexose transporter in Schizosaccharomyces pombe

Proceedings of the National Academy of Sciences of the United States of America
N SauerW Tanner

Abstract

Schizosaccharomyces pombe cells were transformed with an S. pombe expression vector containing a full-length cDNA of the Chlorella hexose transporter. The transformed cells accumulated 3-O-methylglucose up to 10-fold, whereas wild-type S. pombe and control transformants could only equilibrate this sugar analogue. In a pH-jump experiment, in which extracellular pH was lowered by 1.9 units, the accumulation ratio was increased in transformed cells but not in control cells. This result indicates that the gene product, Chlorella H+/glucose-symporter protein, and a pH gradient suffice for active sugar uptake. Km values for glucose, 6-deoxyglucose, and 3-O-methylglucose of 1.5 x 10(-5) M, 2.7 x 10(-4) M, and 1.0 x 10(-3) M, respectively, were identical in Chlorella and in S. pombe cells transformed with Chlorella cDNA and approximately 100-fold lower than those of the endogenous transport system of S. pombe.

References

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Citations

Dec 1, 1994·Plant Molecular Biology·N SauerE Truernit
Mar 1, 1991·Current Genetics·K WolfN Sauer
Feb 1, 1993·Journal of Bioenergetics and Biomembranes·V VacataH Lecar
Jul 13, 2006·Planta·Rainer Hedrich, Irene Marten
Feb 10, 2009·Planta·Ekrem Dündar, Daniel R Bush
Aug 24, 1994·Biochimica Et Biophysica Acta·M OpekarováW Tanner
Oct 18, 1994·Biochimica Et Biophysica Acta·Z S LiS Delrot
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·W. Tanner, T. Caspari
Sep 21, 2004·Annual Review of Plant Biology·Sylvie LalondeWolf B Frommer
Aug 10, 2010·Journal of Photochemistry and Photobiology. B, Biology·An-Long HuHan-Hong Xu
Nov 21, 2014·The FEBS Journal·Petr HermanJan Malinsky
Dec 1, 1991·Bioscience Reports·P J Henderson
Oct 6, 2005·The Journal of Biological Chemistry·Petra Reihl, Jürgen Stolz
May 30, 2012·Plant Biology·I Haferkamp, N Linka
May 15, 2007·The Biochemical Journal·Silke Schilling, Christine Oesterhelt
Oct 24, 2001·Plant & Cell Physiology·T FuruichiS Muto
Jun 4, 2020·Plant Methods·Deepti M NambiarNaveen C Bisht
Aug 28, 2020·Pflügers Archiv : European journal of physiology·Dietmar Geiger
Apr 5, 2000·Biochimica Et Biophysica Acta·M Büttner, N Sauer
Oct 1, 1995·Molecular and Cellular Biology·M E DrewS M Landfear
Feb 15, 2003·Biochimica Et Biophysica Acta·M Opekarová, W Tanner

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