Functional expression of the colonic H+,K+-ATPase alpha-subunit. Pharmacologic properties and assembly with X+,K+-ATPase beta-subunits.

The Journal of Biological Chemistry
Juan CodinaThomas D DuBose

Abstract

The functional and pharmacological properties of the alpha-subunit of the colonic H+,K+-ATPase (alphaC) were studied in Xenopus laevis oocytes. alphaC was injected with different rat beta-subunits, the beta-subunit of the gastric H+,K+-ATPase (betaG, the only H+, K+-ATPase beta-subunit identified in rat), or the beta1-subunit of the Na+,K+-ATPase (beta1) (associated with the basolateral Na+, K+-ATPase, but also expressed in the epithelial apical membranes of rat distal colon) (Marxer, A., Stieger, B., Quarini, A., Kashgarian, M., and Hauri, H. P. (1989) J. Cell Biol. 109, 1057-1069). The effect of the different beta-subunits was studied by measuring 86Rb+ uptake (a K+ congener) in the presence or absence of Sch-28080 and ouabain. Significant Na+-independent 86Rb+ uptake was observed only when alphaC was coexpressed with one of the beta-subunits. The expressed alphaCbeta1 and alphaCbetaG complexes were not inhibited by Sch-28080, were only partially sensitive to ouabain (IC50 = 400-600 microM, in the presence of external 1 mM KCl), and exhibited comparable K+ activation kinetics. Coexpression of alphaC with epitope-tagged betaG or beta1, followed by immunopurification of the alphabeta complexes, confirmed stable assembly of alph...Continue Reading

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