PMID: 8608409Oct 1, 1995Paper

Functional expression of the P-glycoprotein mdr in primary cultures of bovine cerebral capillary endothelial cells

Cell Biology and Toxicology
D Lechardeur, D Scherman

Abstract

The P-glycoprotein mdr is expressed not only in tumoral cells, but also in nontransformed cells, including the specialized endothelial cells of brain capillaries which build up the blood-brain barrier. Since all previously identified blood-brain barrier markers are rapidly lost when cerebral capillary endothelial cells are maintained in primary culture, we have investigated whether P-glycoprotein (P-gp) would follow the same rule, in order to address the influence of the cerebral environment on the specific P-gp expression in the brain endothelium. As compared to freshly isolated purified cerebral capillaries, P-glycoprotein was detected by immunochemistry at a high level in 5-7 day primary cultures. In our culture conditions, P-glycoprotein was immunodetected at a lower molecular weight than that found in freshly isolated capillaries. Enzymatic deglycosylation led to the same 130 kDa protein for both fresh and cultured samples, suggesting that P-gp post-translational modifications were altered in primary cultures. However, studies on the uptake and efflux of the P-gp substrate [3H]vinblastine, and on the effect of various mdr reversing agents on the uptake and efflux, clearly indicated that the efflux pump function of the P-gl...Continue Reading

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Citations

Dec 13, 2000·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·P J GaillardD D Breimer
Nov 20, 1998·Journal of Photochemistry and Photobiology. B, Biology·D KesselA Morgan
Jun 19, 2010·Journal of Veterinary Pharmacology and Therapeutics·D ZahnerE Petzinger
Aug 9, 2013·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Jenni J HakkarainenMarkus M Forsberg
Apr 16, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Rongzi Yan, Eve M Taylor

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