PMID: 3753981Mar 1, 1986Paper

Functional expression of the polymeric immunoglobulin receptor from cloned cDNA in fibroblasts

The Journal of Cell Biology
D L DeitcherK E Mostov

Abstract

The polymeric immunoglobulin receptor, a transmembrane protein, is made by a variety of polarized epithelial cells. After synthesis, the receptor is sent to the basolateral surface where it binds polymeric IgA and IgM. The receptor-ligand complex is endocytosed, transported across the cell in vesicles, and re-exocytosed at the apical surface. At some point the receptor is proteolytically cleaved so that its extracellular ligand binding portion (known as secretory component) is severed from the membrane and released together with the polymeric immunoglobulin at the apical surface. We have used a cDNA clone coding for the rabbit receptor and a retroviral expression system to express the receptor in a nonpolarized mouse fibroblast cell line, psi 2, that normally does not synthesize the receptor. The receptor is glycosylated and sent to the cell surface. The cell cleaves the receptor to a group of polypeptides that are released into the medium and co-migrate with authentic rabbit secretory component. Cleavage and release of secretory component do not depend on the presence of ligand. The cells express on their surface 9,600 binding sites for the ligand, dimeric IgA. The ligand can be rapidly endocytosed and then re-exocytosed, all ...Continue Reading

References

Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E Rodriguez Boulan, D D Sabatini
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R D Cone, R C Mulligan
Jan 1, 1982·Cold Spring Harbor Symposia on Quantitative Biology·M S BrownJ L Goldstein
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M S Bretscher
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G Blobel
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·K E MostovG Blobel

❮ Previous
Next ❯

Citations

Aug 6, 1998·Journal of Immunological Methods·M AsanoI Moro
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B D WalkerJ Sodroski
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A J KormanR C Mulligan
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M A Shia, H F Lodish
Oct 1, 1989·American Journal of Respiratory Cell and Molecular Biology·P P BreitfeldK E Mostov
Jan 1, 1989·Immunological Investigations·W R Brown, T M Kloppel
Jul 24, 2004·Immunology·Masatake AsanoItaru Moro
Sep 26, 2003·Scandinavian Journal of Immunology·N MatsumotoI Moro
May 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W R Brown, T M Kloppel
Jul 20, 1999·The Journal of Biological Chemistry·V A BarrS I Taylor
Apr 1, 2003·The Journal of Biological Chemistry·Joshua H LipschutzKeith E Mostov
Sep 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Annemiek B Van SprielJan G J Van De Winkel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.