Functional expression of the serotonin 5-HT7 receptor in human glioblastoma cell lines

British Journal of Pharmacology
Cécile MahéP Schoeffter

Abstract

Serotonin 5-HT(7) receptors are present in astrocytes. Understanding their role in this type of cell would greatly benefit from the identification of astroglial cell lines expressing this receptor type. The aim of the present study was to assess the expression of native 5-HT(7) receptors and 5-HT(7) receptor mRNA in a number of human glioblastoma cell lines, by means of cAMP measurements, Western blot analysis and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. 5-Hydroxytryptamine (5-HT), 5-carboxamidotryptamine (5-CT), 5-methoxytryptamine (5-MeOT) and 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT) induced concentration-dependent stimulations of cAMP accumulation in the human glioblastoma cell lines, U-373 MG, U-138 MG, U-87 MG, DBTRG-05MG, T98G, H4, CCF-STTG1 and Hs 683. The rank order of potency was 5-CT>5-HT=5-MeOT>8-OH-DPAT. The effect of 5-CT was inhibited in a concentration-dependent manner by the selective 5-HT(7) receptor antagonist SB-269970 in all human glioblastoma cells. Schild analyses yielded slope factors close to unity (0.89-1.13) and pA(2) values of 8.69-9.05. Western blot analysis revealed the presence of immunoreactive bands corresponding to the human 5-HT(7) receptor in extracts of all...Continue Reading

References

May 1, 1995·British Journal of Pharmacology·Z P ToL B Jakeman
Mar 1, 1994·Neuropharmacology·F G Boess, I L Martin
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·N AdhamT A Branchek
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M RuatJ C Schwartz
Jan 1, 1995·Advances in Neuroimmunology·B S Huneycutt, E N Benveniste
Apr 1, 1997·Trends in Pharmacological Sciences·R M EglenG R Martin
Aug 24, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Z CohenE Hamel
May 24, 2000·British Journal of Pharmacology·J J HaganD R Thomas
Mar 14, 2001·The Journal of Comparative Neurology·M A Belenky, G E Pickard
Dec 26, 2001·Neuropharmacology·Catherine H GillCeri H Davies
Mar 1, 1959·British Journal of Pharmacology and Chemotherapy·O ARUNLAKSHANA, H O SCHILD
Oct 3, 2003·Trends in Neurosciences·Bruce RansomMaiken Nedergaard
Feb 18, 2004·Current Drug Targets. CNS and Neurological Disorders·David R Thomas, Jim J Hagan

❮ Previous
Next ❯

Citations

Jun 8, 2013·European Journal of Pharmacology·Abdulmecit AlbayrakAhmet Dogrul
Sep 11, 2008·The Journal of Biological Chemistry·Vaibhav P Pai, Nelson D Horseman
Oct 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Malinda A StullNelson D Horseman
Dec 10, 2009·Pathology Oncology Research : POR·Daniel Pretto SchunemannAdriana Brondani da Rocha
Jul 5, 2005·Neuropharmacology·Cécile MahéPhilippe Schoeffter
Nov 2, 2016·Therapeutic Advances in Medical Oncology·Shahdabul FarazA Gabriella Wernicke
May 23, 2018·Neuro-oncology·Shawn Gillespie, Michelle Monje
Mar 27, 2020·OncoTargets and Therapy·Xiaohui DuJungang Xie
Dec 23, 2020·International Journal of Molecular Sciences·Alessandra TempioMarcello Leopoldo
Mar 1, 2021·Seminars in Cancer Biology·Adam LaukoJustin D Lathia
Jul 19, 2017·Trends in Cancer·Humsa Venkatesh, Michelle Monje
Apr 4, 2021·International Journal of Molecular Sciences·Alba Bellot-SaezYossi Buskila

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.