Abstract
Multidrug resistance (MDR) genes, which are ATP-binding cassette family genes, encode the cell surface glycoprotein, P-glycoprotein, which functions as an energy-dependent drug efflux pump. Two relevant human genes, PGY1 and PGY3, are located on human chromosome 7, and three relevant mouse genes, mdr1a, mdr1b, and mdr2, are located on mouse chromosome 5. An LMD1 cell line was established after the transfer of a 580-kb yeast artificial chromosome (YAC) clone carrying the human MDR locus into mouse L cells; the cell line was shown to have stably integrated YAC DNA in an apparent intact form. Using LMD1 cells as the parental cell line, five vincristine-resistant sublines, designated LMD1-V50, LMD1-V100, LMD1-V200, LMD1-V500, and LMD1-V1000, were isolated by exposure to increasing concentrations of the drug. LMD1-V50, LMD1-V100, LMD1-V200, LMD1-V500, and LMD1-V1000 showed 3-, 7-, 13-, 45-, and 110-fold higher resistance to the cytotoxic effects of vincristine, respectively, than their parental counterpart, LMD1. Immunofluorescence, Western blot, and Northern blot analyses revealed that the human PGY1 gene or its product was overexpressed, accompanied by gene amplification. The human PGY3 gene was also overexpressed in the LMD1-V20,...Continue Reading
References
Sep 16, 1992·Biochemical and Biophysical Research Communications·M MiyazakiM Kuwano
Mar 16, 1992·Biochemical and Biophysical Research Communications·H TanimuraM Kuwano
Jan 1, 1991·DNA Sequence : the Journal of DNA Sequencing and Mapping·J A EndicottV Ling
Jul 15, 1991·Gene·A K Srivastava, D Schlessinger
Apr 1, 1991·Genomics·C HuxleyM V Olson
Aug 1, 1990·Genomics·M D'UrsoD Schlessinger
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·V PachnisF Costantini
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E D Green, M V Olson
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H Hamada, T Tsuruo
Jun 16, 1989·Science·B H BrownsteinM V Olson
Dec 29, 1989·Biochemical and Biophysical Research Communications·K KohnoM Kuwano
Jan 1, 1989·Advances in Cancer Research·A M van der Bliek, P Borst
Aug 4, 1985·Nature·J R RiordanV Ling
Nov 7, 1986·Cell·C J ChenI B Roninson
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C R FairchildK H Cowan
Nov 1, 1988·Japanese Journal of Cancer Research : Gann·K KohnoM Kuwano
Jul 1, 1986·Somatic Cell and Molecular Genetics·A FojoI Pastan
Oct 23, 1986·Nature·P GrosD E Housman
May 2, 1986·Science·D W ShenM M Gottesman
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·I B RoninsonI Pastan
May 28, 1987·The New England Journal of Medicine·I Pastan, M Gottesman
May 29, 1987·Science·S S ThorgeirssonM M Gottesman
Nov 7, 1986·Cell·P GrosD Housman
Mar 1, 1985·Somatic Cell and Molecular Genetics·S AkiyamaM M Gottesman
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Feb 9, 1984·Nature·G M WahlM L DeRose
Jun 14, 1984·Nature·I B RoninsonA Varshavsky
Mar 30, 1994·Biochemical and Biophysical Research Communications·K AsakunoM Kuwano
May 20, 1994·Cell·A H SchinkelH P te Riele
Mar 26, 1993·Science·W M StraussR Jaenisch
Nov 5, 1993·Cell·J J SmitM A van Roon
May 16, 1994·Biochemical and Biophysical Research Communications·M WadaD Schlessinger
May 11, 1994·Nucleic Acids Research·M WadaM Kuwano
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K R PetersonG Stamatoyannopoulos
Aug 1, 1993·Genomics·T Featherstone, C Huxley
Mar 18, 1993·Nature·A SchedlG Schütz
Mar 1, 1993·Somatic Cell and Molecular Genetics·G H Nonet, G M Wahl
Oct 1, 1995·Genome Research·K TorigoeM Wada
Citations
Jul 1, 1997·Somatic Cell and Molecular Genetics·H KusabaM Wada
Jul 20, 1999·European Journal of Biochemistry·H KusabaM Wada
May 15, 1996·Nucleic Acids Research·Y MakinoK Kohno
May 8, 1998·Genomics·K TorigoeM Wada
Aug 28, 2002·The Journal of Pharmacy and Pharmacology·Chika YamamotoYasufumi Sawada
Apr 17, 1999·Biopharmaceutics & Drug Disposition·J MatsuzakiY Sawada
Feb 7, 2001·Blood·J NagayamaM Wada
Aug 3, 2007·American Journal of Physiology. Cell Physiology·Fang XuPravin B Sehgal
Dec 18, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Somshuvra MukhopadhyayPravin B Sehgal
Apr 16, 1998·The Journal of Biological Chemistry·T OhgaK Kohno
Nov 14, 1998·Biochemical and Biophysical Research Communications·T UchiumiM Kuwano