Functional F11L and K1L genes in modified vaccinia virus Ankara restore virus-induced cell motility but not growth in human and murine cells

Virology
Joachim ZwillingGerd Sutter

Abstract

Modified vaccinia virus Ankara (MVA) was generated by serial passaging in chicken embryo fibroblasts. During this attenuation, MVA lost the capacity to productively grow in human and most other mammalian cell lines, as well as acquiring a multitude of deletions and mutations in the MVA genome. This means that the precise molecular basis for the MVA host-range restriction is still unknown. The vaccinia virus (VACV) genes F11L and K1L are mutated or truncated in MVA. F11L was previously implicated in VACV-induced cell motility and virion maturation. Here, we demonstrate that the restoration of F11L gene expression in MVA rescued virus-induced cell motility, but had no impact on MVA virion maturation and host-range restriction. Additional insertion of the K1L gene, which restores MVA replication in RK-13 cells, was not sufficient to extend MVA growth capacity to other mammalian cells.

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G Sutter, B Moss
Nov 1, 1990·Virology·M E PerkusE Paoletti
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·S Lin, S S Broyles
Aug 13, 2003·The Journal of Biological Chemistry·Julie G Donaldson
Oct 8, 2003·Current Drug Targets. Infectious Disorders·Gerd Sutter, Caroline Staib
May 20, 2008·Traffic·Ivonne MoralesJacomine Krijnse Locker

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Citations

Apr 20, 2012·Journal of Virology·Chad R Irwin, David H Evans
Aug 3, 2011·Expert Review of Vaccines·Joseph W Golden, Jay W Hooper
Jan 5, 2011·Immunological Reviews·Bernard Moss
May 25, 2012·The Journal of General Virology·Virginie DoceulGeoffrey L Smith
Jan 17, 2014·Journal of Virology·Ismar R HagaPhilippa M Beard

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