Functional Genes and Bacterial Communities During Organohalide Respiration of Chloroethenes in Microcosms of Multi-Contaminated Groundwater

Frontiers in Microbiology
Louis HermonCatherine Joulian

Abstract

Microcosm experiments with CE-contaminated groundwater from a former industrial site were set-up to evaluate the relationships between biological CE dissipation, dehalogenase genes abundance and bacterial genera diversity. Impact of high concentrations of PCE on organohalide respiration was also evaluated. Complete or partial dechlorination of PCE, TCE, cis-DCE and VC was observed independently of the addition of a reducing agent (Na2S) or an electron donor (acetate). The addition of either 10 or 100 μM PCE had no effect on organohalide respiration. qPCR analysis of reductive dehalogenases genes (pceA, tceA, vcrA, and bvcA) indicated that the version of pceA gene found in the genus Dehalococcoides [hereafter named pceA(Dhc)] and vcrA gene increased in abundance by one order of magnitude during the first 10 days of incubation. The version of the pceA gene found, among others, in the genus Dehalobacter, Sulfurospirillum, Desulfuromonas, and Geobacter [hereafter named pceA(Dhb)] and bvcA gene showed very low abundance. The tceA gene was not detected throughout the experiment. The proportion of pceA(Dhc) or vcrA genes relative to the universal 16S ribosomal RNA (16S rRNA) gene increased by up to 6-fold upon completion of cis-DCE di...Continue Reading

References

Oct 1, 1991·Applied and Environmental Microbiology·D L Freedman, J M Gossett
Oct 15, 1994·FEMS Microbiology Letters·D P Terzenbach, M Blaut
Jul 2, 1999·Applied and Environmental Microbiology·X Maymó-GatellS H Zinder
Feb 12, 2000·Applied and Environmental Microbiology·J K MagnusonD R Burris
Apr 1, 2000·Applied and Environmental Microbiology·F E LöfflerJ M Tiedje
May 16, 2001·Environmental Science & Technology·X Maymó-GatellS H Zinder
Feb 2, 2002·Applied and Environmental Microbiology·Edwin R HendricksonRichard C Ebersole
Mar 28, 2002·Applied and Environmental Microbiology·Jutta KleikemperJosef Zeyer
Jun 12, 2002·Journal of Bacteriology·Akiko SuyamaKensuke Furukawa
Aug 16, 2002·Journal of Contaminant Hydrology·Ralph E Beeman, Charles A Bleckmann
May 12, 2004·Journal of Microbiological Methods·Juan C López-GutiérrezLaurent Philippot
Sep 4, 2004·Applied and Environmental Microbiology·Tina HölscherLorenz Adrian
Oct 7, 2004·Applied and Environmental Microbiology·Rosa Krajmalnik-BrownFrank E Löffler
Mar 7, 2006·Applied and Environmental Microbiology·Youlboong SungFrank E Löffler
Apr 7, 2006·Applied and Environmental Microbiology·Youlboong SungFrank E Löffler
Jul 6, 2006·Applied and Environmental Microbiology·T Z DeSantisG L Andersen
Nov 23, 2006·FEMS Microbiology Ecology·Melanie Duhamel, Elizabeth A Edwards
Mar 6, 2007·Applied and Environmental Microbiology·Jianzhong HeLisa Alvarez-Cohen
Apr 19, 2007·Environmental Science & Technology·Rebecca C DapratoJoseph B Hughes
Sep 26, 2007·FEMS Microbiology Ecology·Alban Ramette
Feb 28, 2008·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Taiki FutagamiKensuke Furukawa
May 12, 2009·Advances in Applied Microbiology·Larry L Barton, Guy D Fauque
Jul 17, 2009·Environmental Science & Technology·Concepción Carreón-DiazcontiMark L Brusseau
Mar 6, 2010·Trends in Microbiology·Yin Chen, J Colin Murrell
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Jun 15, 2010·Environmental Science & Technology·Farai MaphosaWilfred F M Röling
Aug 17, 2010·Bioinformatics·Robert C Edgar

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Citations

Jan 14, 2021·Environmental Science and Pollution Research International·Daniele GhezziStefano Fedi
Sep 15, 2020·The Journal of Allergy and Clinical Immunology·Louise-Eva VandenborghtLaurence Delhaes
Dec 4, 2021·Water Environment Research : a Research Publication of the Water Environment Federation·Wei-Ting ChenChih-Ming Kao

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Datasets Mentioned

BETA
SRP160122
MK312632

Methods Mentioned

BETA
ionic chromatography
PCR
electrophoresis
GenoScreen
PCA

Software Mentioned

Usearch
Bionumerics
QIIME
Metabiote Online
lite PERL
R
BioNJ
Uclust
FLASH
CutAdapt

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