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Functional genetic screens for enhancer elements in the human genome using CRISPR-Cas9

Nature Biotechnology

Jan 12, 2016

Gozde KorkmazReuven Agami

Abstract

Systematic identification of noncoding regulatory elements has, to date, mainly relied on large-scale reporter assays that do not reproduce endogenous conditions. We present two distinct CRISPR-Cas9 genetic screens to identify and characterize functional enhancers in their native contex...read more

Mentioned in this Paper

TP53 gene
Genome
Estrogen receptor alpha, human
CRISPR-Cas Systems
ESR1
Genetic Screening (Procedure)
Genomic Screening
TP53
MCF-7 Cells
ESR1 gene
136
2
3
698
Paper Details
References
  • References38
  • Citations72
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Functional genetic screens for enhancer elements in the human genome using CRISPR-Cas9

Nature Biotechnology

Jan 12, 2016

Gozde KorkmazReuven Agami

PMID: 26751173

DOI: 10.1038/nbt.3450

Abstract

Systematic identification of noncoding regulatory elements has, to date, mainly relied on large-scale reporter assays that do not reproduce endogenous conditions. We present two distinct CRISPR-Cas9 genetic screens to identify and characterize functional enhancers in their native contex...read more

Mentioned in this Paper

TP53 gene
Genome
Estrogen receptor alpha, human
CRISPR-Cas Systems
ESR1
Genetic Screening (Procedure)
Genomic Screening
TP53
MCF-7 Cells
ESR1 gene
136
2
3
698

Similar Papers Found In These Feeds

Related Papers

Paper Details
References
  • References38
  • Citations72
12345...
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