Functional genomics identifies regulators of the phototransduction machinery in the Drosophila larval eye and adult ocelli

Developmental Biology
Abhishek Kumar MishraSimon G Sprecher

Abstract

Sensory perception of light is mediated by specialized Photoreceptor neurons (PRs) in the eye. During development all PRs are genetically determined to express a specific Rhodopsin (Rh) gene and genes mediating a functional phototransduction pathway. While the genetic and molecular mechanisms of PR development is well described in the adult compound eye, it remains unclear how the expression of Rhodopsins and the phototransduction cascade is regulated in other visual organs in Drosophila, such as the larval eye and adult ocelli. Using transcriptome analysis of larval PR-subtypes and ocellar PRs we identify and study new regulators required during PR differentiation or necessary for the expression of specific signaling molecules of the functional phototransduction pathway. We found that the transcription factor Krüppel (Kr) is enriched in the larval eye and controls PR differentiation by promoting Rh5 and Rh6 expression. We also identified Camta, Lola, Dve and Hazy as key genes acting during ocellar PR differentiation. Further we show that these transcriptional regulators control gene expression of the phototransduction cascade in both larval eye and adult ocelli. Our results show that PR cell type-specific transcriptome profili...Continue Reading

References

Sep 1, 1993·Cell and Tissue Research·P GreenV Hartenstein
Jan 10, 1996·Developmental Biology·E R VandendriesR Reinke
Jul 31, 1998·Development Genes and Evolution·D KosmanJ Reinitz
May 10, 2001·Trends in Biochemical Sciences·T OkadaK P Hofmann
May 31, 2001·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·M K KerrG A Churchill
Aug 10, 2001·Current Opinion in Structural Biology·T Okada, K Palczewski
Sep 17, 2002·Genes & Development·Jose BessaRichard S Mann
Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Lydia MichautWalter J Gehring
Aug 11, 2004·Developmental Biology·Pedro M DomingosBertrand Mollereau
Jun 2, 2005·Developmental Biology·Toshie KaiAllan C Spradling
Oct 6, 2005·Nucleic Acids Research·Zhiyong YangRonald L Davis
Jun 8, 2006·Annual Review of Biochemistry·Krzysztof Palczewski
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Johannes BischofKonrad Basler
Sep 6, 2007·Genes & Development·Simon G SprecherClaude Desplan
Dec 7, 2007·Mechanisms of Development·Limin Zheng, Richard W Carthew
Jan 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Stuart J ElgarMichael V Taylor

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Citations

Oct 21, 2016·Genesis : the Journal of Genetics and Development·Edwin Chaharbakhshi, Jennifer C Jemc
Jul 13, 2019·PLoS Genetics·Cornelia FritschSimon G Sprecher
Nov 25, 2018·International Journal of Molecular Sciences·Zhaojiang GuoYoujun Zhang
Aug 8, 2017·ELife·Ivan LarderetSimon G Sprecher
Oct 22, 2020·Proceedings. Biological Sciences·J Arvid ÅgrenAndrew G Clark
Jul 28, 2021·PLoS Genetics·Abhishek Kumar MishraSimon G Sprecher

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