Functional heterogeneity of murine intestinal intraepithelial lymphocytes: studies using TCR-alpha beta+ IEL lines and fresh IEL isolates reveal multiple cytotoxic subsets differentiated by CD5, CD8 alpha/alpha, and CD8 alpha/beta expression

Developmental and Comparative Immunology
R L MosleyJ R Klein

Abstract

Three T-cell lines, isolated from murine small intestine epithelia, have been studied with respect to phenotypic properties and cytotoxic activity. All lines were TCR-alpha beta+, Thy-1+, CD3+, CD4-, CD8+ but differed in that one line was CD8 alpha/alpha+, CD5-; one line was CD8 alpha/alpha+, CD5+; and one line was CD8 alpha/beta+, CD5+. Both the CD8 alpha/alpha+, CD5-, and the CD8 alpha/beta+, CD5+ lines lysed antigen-bearing target cells; however, the latter line also spontaneously lysed natural killer (NK)-sensitive target cells. The CD8 alpha/alpha+, CD5+ IEL line was nonlytic for antigen-bearing target cells, for NK-sensitive target cells, and in assays that detect lytic activity regardless of specificity. Three-color flow cytometric analyses of intestinal intraepithelial lymphocytes (IEL) in freshly-extracted preparations indicated that cells with the phenotypes of the three IEL lines are normally present in the murine intestine epithelium, and revealed considerable variability in the distribution and density of CD5 expression on murine IEL. In freshly extracted IEL depleted of CD5 or CD8 beta by cell sorting, cytotoxicity was found to reside both within the CD5+ and the CD5- IEL subsets, as well as in the CD8 beta-deplet...Continue Reading

References

Nov 25, 1992·Journal of Immunological Methods·R L Mosley, J R Klein
Jul 1, 1990·European Journal of Immunology·J L VineyT T MacDonald
May 1, 1988·The Journal of Clinical Investigation·B E BiererB R Smith
Feb 1, 1985·Immunology Today·P B ErnstJ Bienenstock

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Citations

Jan 1, 1997·Springer Seminars in Immunopathology·H ScottP Brandtzaeg
Jul 13, 2000·Veterinary Immunology and Immunopathology·P RoccabiancaP F Moore
Jan 14, 2000·Journal of Pediatric Gastroenterology and Nutrition·L Mayer
Aug 18, 2004·Trends in Molecular Medicine·Tracy HussellAndrew E Williams
Oct 31, 2001·Nature Immunology·A HaydayJ Shires

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