Nov 4, 2018

Functional heterogeneity within the developing zebrafish epicardium

BioRxiv : the Preprint Server for Biology
Michael WeinbergerPaul R. Riley

Abstract

The epicardium is essential during cardiac development, homeostasis and repair and yet fundamental insights into its underlying cell biology, notably epicardium formation, lineage heterogeneity and functional cross-talk with other cell types in the heart, are currently lacking. In this study, we investigated epicardial heterogeneity and the functional diversity of discrete epicardial subpopulations in the developing zebrafish heart. Single-cell RNA-sequencing uncovered three epicardial subpopulations with specific genetic programmes and distinctive spatial distribution within the developing heart. Perturbation of unique gene signatures uncovered distinct functions associated with each subpopulation and established novel epicardial roles in cell adhesion, migration, and chemotaxis as a mechanism for recruitment of leukocytes into the heart. This work elucidates the mutual spatiotemporal relationships between different epicardial subpopulations and assigns unique function to each during cardiac development. Understanding which mechanisms cells employ to establish a functional epicardium and to communicate with other cardiovascular cell types during development will bring us closer to repairing cellular relationships that are disr...Continue Reading

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Mentioned in this Paper

Study
Cross-talk
Genes
Sequence Determinations, RNA
Spatial Distribution
White Blood Cell Count Procedure
Chemotaxis
Cardiovascular Diseases
Genetics (Procedure)
Leukocytes

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