PMID: 2492493Jan 1, 1989Paper

Functional homology of chemotactic methylesterases from Bacillus subtilis and Escherichia coli

Journal of Bacteriology
D O Nettleton, G W Ordal

Abstract

The methylesterase enzyme from Bacillus subtilis was compared with that from Escherichia coli. Both enzymes were able to demethylate methyl-accepting chemotaxis proteins (MCPs) from the other organism and were similarly affected by variations in glycerol, magnesium ion, or pH. When attractants were added to a mixture of B. subtilis MCPs and E. coli methylesterase, the rate of demethylation was enhanced. Conversely, when attractants were added to a mixture of E. coli MCPs and B. subtilis methylesterase, the rate of demethylation was diminished. These effects are what would be expected if, in these in vitro systems, the MCPs determined the rate of demethylation. These data suggest that, although the enzymes are from evolutionarily divergent organisms and are different in size, they have considerable functional homology.

References

Jan 1, 1977·Journal of Bacteriology·G W Ordal, K J Gibson
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·M S SpringerJ Adler
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·M F GoyJ Adler
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M L ToewsJ Adler
Jan 1, 1979·Current Topics in Cellular Regulation·E Schiffmann, J I Gallin
Jan 1, 1977·Journal of Bacteriology·G W OrdalK J Gibson
Jan 1, 1976·Annual Review of Microbiology·I Chet, R Mitchell
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E N KortJ Adler
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Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D Sherris, J S Parkinson
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Jan 1, 1981·Annual Review of Biochemistry·D E Koshland

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Citations

May 29, 2000·The Journal of Biological Chemistry·M A ZimmerG W Ordal
Jan 21, 2004·Molecular Microbiology·Michael W Bunn, George W Ordal
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Hendrik Szurmant, George W Ordal
Jan 1, 1995·Pharmacology & Therapeutics·L F Garrity, G W Ordal
Feb 1, 1990·Journal of Bacteriology·M S ThoelkeG W Ordal

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