PMID: 9420230Jan 7, 1998Paper

Functional identification and analysis of cis-acting sequences which mediate genome cleavage and packaging in human herpesvirus 6

Journal of Virology
Hongyu Deng, S Dewhurst

Abstract

Sequences present at the genomic termini of herpesviruses become linked during lytic-phase replication and provide the substrate for cleavage and packaging of unit length viral genomes. We have previously shown that homologs of the consensus herpesvirus cleavage-packaging signals, pac1 and pac2, are located at the left and right genomic termini of human herpesvirus 6 (HHV-6), respectively. Immediately adjacent to these elements are two distinct arrays of human telomeric repeat sequences (TRS). We now show that the unique sequence element formed at the junction of HHV-6B genome concatemers (pac2-pac1) is necessary and sufficient for virally mediated cleavage of plasmid DNAs containing the HHV-6B lytic-phase origin of DNA replication (oriLyt). The concatemeric junction sequence also allowed for the packaging of these plasmid molecules into intracellular nucleocapsids as well as mature, infectious viral particles. In addition, this element significantly enhanced the replication efficiency of oriLyt-containing plasmids in virally infected cells. Experiments revealed that the concatemeric junction sequence possesses an unusual, S1 nuclease-sensitive conformation (anisomorphic DNA), which might play a role in this apparent enhancemen...Continue Reading

References

Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·F WohlrabR D Wells
Jan 1, 1991·The Journal of General Virology·K ShirakiM Takahashi
Nov 1, 1966·Journal of Bacteriology·M Benyesh-MelnickV Vonka
Jun 14, 1967·Journal of Molecular Biology·B Hirt
Jan 1, 1980·Annals of the New York Academy of Sciences·N FrenkelD A Vlazny
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D A VlaznyN Frenkel
Jul 15, 1993·The New England Journal of Medicine·R W ConeL Corey
Sep 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J A McCullersR J Whitley
May 1, 1996·Journal of Virology·A SeveriniD L Tyrrell

❮ Previous
Next ❯

Citations

Jan 18, 2005·Clinical Microbiology Reviews·Leen De BolleErik De Clercq
Sep 17, 2010·Journal of Virology·Guillaume Morissette, Louis Flamand
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·K AdelmanJ D Baines
Dec 19, 2002·Journal of Medical Microbiology·D H Dockrell
Feb 27, 2004·British Journal of Haematology·Tetsushi Yoshikawa
May 5, 2017·Journal of Virology·Shella Gilbert-GirardLouis Flamand
Dec 6, 2019·Viruses·Joachim DennerBenedikt B Kaufer
May 18, 2000·Reviews in Medical Virology·D A Clark
Dec 7, 2010·Virology·Shaun TylerR Eberle

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.