Functional Identification and Characterization of Genes Cloned from Halophyte Seashore Paspalum Conferring Salinity and Cadmium Tolerance

Frontiers in Plant Science
Yu ChenBingru Huang

Abstract

Salinity-affected and heavy metal-contaminated soils limit the growth of glycophytic plants. Identifying genes responsible for superior tolerance to salinity and heavy metals in halophytes has great potential for use in developing salinity- and Cd-tolerant glycophytes. The objective of this study was to identify salinity- and Cd-tolerance related genes in seashore paspalum (Paspalum vaginatum), a halophytic perennial grass species, using yeast cDNA expression library screening method. Based on the Gateway-compatible vector system, a high-quality entry library was constructed, which contained 9.9 × 10(6) clones with an average inserted fragment length of 1.48 kb representing a 100% full-length rate. The yeast expression libraries were screened in a salinity-sensitive and a Cd-sensitive yeast mutant. The screening yielded 32 salinity-tolerant clones harboring 18 salinity-tolerance genes and 20 Cd-tolerant clones, including five Cd-tolerance genes. qPCR analysis confirmed that most of the 18 salinity-tolerance and five Cd-tolerance genes were up-regulated at the transcript level in response to salinity or Cd stress in seashore paspalum. Functional analysis indicated that salinity-tolerance genes from seashore paspalum could be inv...Continue Reading

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Citations

Jun 22, 2017·International Journal of Molecular Sciences·Yu LiuBingru Huang
Jul 1, 2017·Frontiers in Plant Science·Hosam O ElansaryMohamed S Elshikh
May 6, 2019·International Journal of Molecular Sciences·Antonella LocascioLynne Yenush
Jul 5, 2018·Frontiers in Plant Science·Ganesh C Nikalje, Penna Suprasanna
Nov 8, 2018·International Journal of Molecular Sciences·Mei ZhangShu-Guang Jian

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Datasets Mentioned

BETA
KU049721
KT203436

Methods Mentioned

BETA
genetic modification
electrophoresis
PCR

Software Mentioned

BLASTX
Primer Premier
SAS9

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