PMID: 8944656Nov 1, 1996Paper

Functional identification of a sarcolemmal chloride channel from bovine tracheal smooth muscle

The American Journal of Physiology
Dany SalvailEric Rousseau

Abstract

The biophysical and pharmacological characteristics of unitary Cl- currents from bovine tracheal smooth muscle cells were studied after reconstitution of microsomal vesicles into planar lipid bilayers. Two types of currents were recorded simultaneously in KCl buffer: the well-defined Ca(2+)-dependent K+ conductance [GK(Ca)] and a much smaller Cl- current, indicating that the Cl- channels under scrutiny originate from the same membrane as the GK(Ca)-type channels, the plasma membrane of airway smooth muscle (ASM) cells. The GK(Ca) activities were eliminated by the use of CsCl buffer. The average unitary Cl- conductance measured in 50 mM trans-250 mM cis CsCl was 77 +/- 6 pS (n = 21), and the reversal potential measured in various CsCl gradients followed the Cl- equilibrium potential as determined from the Nernst equation. In contrast with the previous reports describing the Ca2+ sensitivity of macroscopic ASM Cl- currents, this channel was found to be insensitive to cytoplasmic and extracellular Ca2+ levels. Phosphorylation cocktails, including protein kinases A, G, or C, did not alter the activity of the channel nor did changes in pH. Among a series of Cl- channel inhibitors, 4,4'-diisothiocyanostilbene-2, 2'-disulfonic acid [5...Continue Reading

Citations

Feb 8, 2002·American Journal of Physiology. Cell Physiology·Dany SalvailEric Rousseau
Sep 5, 1998·The American Journal of Physiology·D SalvailE Rousseau
May 11, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Luke J Janssen

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